Stephan HackerAnother great session of #PosterPitches at #NWOCHAINS. We learned about a huge diversity of topics including #SupramoecularAssemblies, #biomarker #sensing, #Ion-selective #membranes, radical #polymerization and #MassSpectrometry.
Stephan HackerAnother great session of #PosterPitches at #NWOCHAINS. We learned about a huge diversity of topics including #SupramoecularAssemblies, #biomarker #sensing, #Ion-selective #membranes, radical #polymerization and #MassSpectrometry.
John Vaccaro (johniac)SciTech Chronicles. . . . . . . . .May 24th, 2025
#metabolic #"Peganum harmala" #sensorial #rue #"cis-urocanic acid" #"Staphylococcus epidermidis" #urocanase #microbiome #nanofiltration #membranes #hydroxide #carbonate #"Venus flytrap" #"aquatic waterwheel" #shading #"striped bug" #"USS F-1" #WHOI #Alvin #Sentry
PLOS Biology#Staphylococcus secretes toxins such as PVL to kill immune cells, incl macrophages. This study shows that PVL binds anionic #phospholipids under acidic conditions, targeting lysosomal & mitochondrial #membranes (but not plasma membrane) to promote bacterial escape @PLOSBiology https://plos.io/3RvcArh
Staphylococcal toxin PVL ruptures model membranes under acidic conditions through interactions with cardiolipin and phosphatidic acid
Staphylococcus aureus secretes toxins, such as Panton-Valentine leukocidin (PVL), to kill immune cells, including macrophages. This study shows that PVL binds phosphatidic acid and cardiolipin in acidic conditions, targeting lysosomal and mitochondrial membranes (but not the plasma membrane) to promote bacterial escape.
Victoria Stuart 🇨🇦 🏳️⚧️🤯 Noise-guided tuning of synthetic protein waves in living cells 😀
https://www.biorxiv.org/content/10.1101/2025.03.21.644572v1
* yeast spatiotemporal protein circuits
* genetically encoded oscillators
* clonal populations oscillate at diff. frequencies
* independ. tunable frequency/amplitude w.i. single population
#Saccharomyces #SyntheticBiology #WaveformEngineering #BioOscillators #Membranes #wafeforms
Noise-guided tuning of synthetic protein waves in living cells
Biological systems use protein circuits to organize cellular activities in space and time, but engineering synthetic dynamics is challenging due to stochastic effects of genetic and biochemical variation on circuit behavior. Genetically encoded oscillators (GEOs) built from bacterial MinDE-family ATPase and Activator modules generate fast orthogonal protein waves in eukaryotic cells, providing an experimental model system for genetic and biochemical coordination of synthetic protein dynamics. Here, we use budding yeast to experimentally define and model phase portraits that reveal how the breadth of frequencies and amplitudes available to a GEO are genetically controlled by ATPase and Activator expression levels and noise. GEO amplitude is encoded by ATPase absolute abundance, making it sensitive to extrinsic noise on a population level. In contrast, GEO frequency is remarkably stable because it is controlled by the Activator:ATPase ratio and thus affected primarily by intrinsic noise. These features facilitate noise-guided design of different expression strategies that act as filters on GEO waveform, enabling us to construct clonal populations that oscillate at different frequencies as well as independently tune frequency and amplitude variation within a single population. By characterizing 169 biochemically distinct GEOs, we provide a rich assortment of phase portraits as starting points for application of our waveform engineering approach. Our findings suggest noise-guided design may be a valuable strategy for achieving precision control over dynamic protein circuits. ### Competing Interest Statement The authors have declared no competing interest.
preLightsA new preLight has appeared! ✨🔔
Barbora Knotkova highlights a preprinted study from Friederike Benning & team (ChaoLab) in which the structure-function relationship at the site of cellular respiration is under review. 👀
CellBioNewsSeeing more in expansion #microscopy: New methods light up #lipid #membranes and allow for high-resolution #protein mapping
https://phys.org/news/2025-03-expansion-microscopy-methods-lipid-membranes.html
Seeing more in expansion microscopy: New methods light up lipid membranes and allow for high-resolution protein mapping
In biology, seeing can lead to understanding, and researchers in Professor Edward Boyden's lab at the McGovern Institute for Brain Research are committed to bringing life into sharper focus. With a pair of new methods, they are expanding the capabilities of expansion microscopy—a high-resolution imaging technique the group introduced in 2015—so researchers everywhere can see more when they look at cells and tissues under a light microscope.
#Quinoa's #stress resistance linked to 10 key #genes
#Chenopodium #genomics #agricuture #drought #food_security #sodium #hydrogen #antiporter #NHX_genes #CPA1 #membranes
https://phys.org/news/2025-03-quinoa-stress-resistance-linked-key.html
Quinoa's stress resistance linked to 10 key genes
In a recent study published in the journal Genes, our team has identified and characterized 10 sodium/hydrogen antiporter (NHX) genes from the quinoa genome that play crucial roles in this remarkable crop's ability to withstand harsh environmental conditions. This discovery provides significant insights into why quinoa can thrive in conditions that would devastate most other food crops.
Markus OsterhoffTag zwo bei den #CMWS #WaterDays.
– Petr Slavíček¹, Prag: #Electrons in water: birth, transport and reactivity
– Anthony Ferté, DESY: Electron impact excitation and ionization of liquid #water
– Nicolas Velasquez², Berlin: Ultrafast #ChargeTransfer in aqueous L-cysteine
– Svenja Hövelmann, Kiel: #XRay #scattering studies of light induced dynamics at liquid interfaces and
biological #membranes
¹ Akzente ergänzt
² vollständiger korrekter Name: Nicolás Sebastián Velásquez González
Luther TownsendThe #performance of #waterproofing #membranes is influenced by its constituent material, how it’s installed, and its compatibility with the base surface it’s applied to. 🛠️💧 These membranes effectively prevent both liquid water and water vapour from penetrating the elements of a building. 🏢🚫💦 https://www.introinto.com.au/intro-to-waterproofing-membranes-types-applications-and-standards/
