Amelia Cervera π§¬Real-time tracking of mRNP complex assembly reveals various mechanisms that synergistically enhance translation repression.
#RNA #Translation #mRNP #Drosophila #DosageCompensation #Preprint
Real-time tracking of mRNP complex assembly reveals various mechanisms that synergistically enhance translation repression
Protein biosynthesis must be highly regulated to ensure proper spatiotemporal gene expression and thus cellular viability. Translation is often modulated at the initiation stage by RNA binding proteins through either promotion or repression of ribosome recruitment to the mRNA. However, it largely remains unknown how the kinetics of mRNA ribonucleoprotein (mRNP) assembly on untranslated regions (UTRs) relates to its translation regulation activity. Using Sex-lethal (Sxl)-mediated translation repression of msl-2 in female fly dosage compensation as a model system, we show that different mechanisms in mRNP assembly synergistically achieve tight translation repression. Using multi-color single-molecule fluorescence microscopy we show that 1) Sxl targets its binding sites via sliding and double-binding, 2) that Unr recruitment is accelerated over 500-fold by RNA-bound Sxl and 3) that Hrp48 further stabilizes RNA-bound Sxl indirectly via ATP-independent RNA remodeling. Overall, we provide a framework to study how multiple RBPs dynamically cooperate with RNA to achieve function. ### Competing Interest Statement The authors have declared no competing interest.
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Gal Haimovich π·π¬β£π³οΈβππ³οΈββ§
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