0 likes, 0 comments - drhowardsmithreports on May 22, 2025: "Growing New Insulin-Producing Pancreas Cells For Diabetics Diabetics with type 1 disease, that is insulin-dependent, must receive insulin injections for the rest of their lives since their own pancreatic tissue fails to produce sufficient quantities of this vital hormone. Efforts to solve this problem include whole organ pancreas transplants and transplants of the insulin-producing beta pancreatic islet cells. One problem is a large enough supply of these beta islet cells. Enter endocrinologists and regenerative medicine researchers at the Netherland’s Leiden University who have developed methods to better isolate large numbers of stem cells that have been transformed into the insulin-producing beta islet cells. Their studies are now published in the journal Science Translational Medicine. Their technique employs density gravity centrifuges to optimally isolate just those stem-cell derived islet cells that will capably produce insulin once transplanted into their human recipients. Their system has undergone successful preclinical trials in diabetic mice. It is more effective and efficient than other cell sorting techniques, and it can be scaled up to produce the large qualities of beta islet cells that will be required for human trials. https://www.science.org/doi/10.1126/scitranslmed.adl4390 https://medicalxpress.com/news/2025-04-technique-architecture-insulin-islet-cells.html#google_vignette #diabetes #insulin #stemcells #betaisletcells #centrifugation".
Blood plasma separation is a prerequisite in numerous biomedical assays involving low abundance plasma-borne biomarkers and thus is the fundamental step before many bioanalytical steps. High-capacity refrigerated centrifuges, which have the advantage of handling large volumes of blood samples, are widely utilized, but they are bulky, non-transportable, and prohibitively expensive for low-resource settings, with prices starting at $1,500. On the other hand, there are low-cost commercial and open-source micro-centrifuges available, but they are incapable of handling typical clinical amounts of blood samples (2-10mL). There is currently no low-cost CE marked centrifuge that can process large volumes of clinical blood samples on the market. As a solution, we customised the rotor of a commercially available low-cost micro-centrifuge (~$125) using 3D printing to enable centrifugation of large clinical blood samples in resource poor-settings. Our custom adaptor ($15) can hold two 9 mL S-Monovette tubes and achieve the same separation performance (yield, cell count, hemolysis, albumin levels) as the control benchtop refrigerated centrifuge, and even outperformed the control in platelet separation by at least four times. This low-cost open-source centrifugation system capable of processing clinical blood tubes could be valuable to low-resource settings where centrifugation is required immediately after blood withdrawal for further testing.
@pwilmart This method for plasma fractionation is one of those that makes me say to myself ... why didn't I think of that!
Rather than remove all the stuff from the house to find the car keys, they spin down the house and look at the bottom of the tube for them, and there they are.
https://doi.org/10.1002/pmic.202200039
#plasma #proteomics #teammassspec #centrifugation #EV #vesicles #EVOSep #SPE
@neely also works on blood proteomics I think.
If N/C ratio is sensed globally, then #centrifugation should not alter developmental timing.
In contrast, if the N/C ratio is sensed locally, then centrifuged coenocytes with tighter nuclear distribution at the cortex may complete #cellularization earlier ?
BioResire
Howard Smith MD, AM
Bose-Einstein-Kondensat
Brian Hampton
DudinLab