Francesca Bottanelli

@[email protected]
356 Followers
295 Following
116 Posts
Asst Prof FU Berlin Biochemistry #superres #microscopy #CRISPR #membrane trafficking aficionado #academicmum of 2
Show threadFrancesca BottanelliFeb 21, 2024
@MCDuncanLab interesting! We def made the switch to EtOH but did not know you can remove everything!
Francesca BottanelliFeb 21, 2024
MCDuncanLabFeb 21, 2024

PSA for #westernblots

Did you know you can often leave out the methanol and SDS and get an excellent transfer?

Our recipe for 4 liters of 10 x is 560g Glycine, 120g Tris Base.

We transfer at 0.5mA for 30 min for most proteins 40 for >150kDa in a biorad rig.

We use an ice block but no stir bar. It gets hot so don't go longer than 40 min.

#Biochemistry #WesternBlotHelp

Francesca BottanelliFeb 21, 2024

#MembraneTrafficking seminars are back in 2024! Join Felix Campelo, Ishier Raote and me next week for two exciting talks. Looking forward to seeing all you membrane enthusiasts again!

Feb 29, 5.00pm CET, 11.00am ET

Francesca BottanelliMay 5, 2023
RT @BarnabaCarlo
Here we are again! @DavidGBroadbent @jenscs83 #autophagy @AIM_autophagy @autophagyuk @AutophagyPapers @WAutophagy https://twitter.com/jcellbiol/status/1654520138190692352
JCellBiol on Twitter

“Quantitative analysis of #autophagy reveals the role of ATG9 and ATG2 in autophagosome formation, say David Broadbent @DavidGBroadbent, Carlo Barnaba @BarnabaCarlo, Gloria Perez, & Jens Schmidt @jenscs83 https://t.co/Z5xeW26met”

Twitter
Francesca BottanelliMay 5, 2023
RT @JCellBiol
STED imaging of endogenously tagged ARF #GTPases reveals their distinct nanoscale localizations, say Luis Wong-Dilworth @FreeThePipette, Francesca Bottanelli @fran_bottanelli and colleagues
#microscopy
Francesca BottanelliApr 28, 2023
RT @RockUPress
New in @JCellBiol: @FreeThePipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases in living cells https://bit.ly/3HhBHcp
STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations

Wong-Dilworth et al. combine CRISPR-Cas9 gene editing with STED super-resolution microscopy to reveal the specific localization of ARF GTPases on different intr

Rockefeller University Press
Show threadFrancesca BottanelliApr 27, 2023
@MCDuncanLab @JCellBiol thank you!!! So nice to finally see the fruit of the hard work of my first PhD student after 2 babies and a pandemic 🥹 he (and all the others who supported!) did such an amazing job
Francesca BottanelliApr 27, 2023
A tour the force of #geneediting and #STED. We have followed up on some of these findings and have 2 papers that will drop soon! looking at endogenous proteins at high resolution in their own unperturbed cellular environment is helping to reveal important trafficking mechanisms!
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RT @JCellBiol
.@FreeThePipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localizati…
https://twitter.com/JCellBiol/status/1651620746806820872
JCellBiol on Twitter

“.@freethepipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases in living cells https://t.co/2rwNuGu0Iz”

Twitter
Show threadFrancesca BottanelliApr 27, 2023
zoom link on here https://felixcampelo.wixsite.com/membranetrafficking @felixmendu @ishier_raote
MEMBRANE TRAFFICKING | Membrane Trafficking

Membrane Trafficking
Francesca BottanelliApr 27, 2023

Do not miss today's session with @IoannouLab talking about autolysosomal exocytosis in neurons (https://rupress.org/jcb/article-abstract/222/6/e202207130/214014/Autolysosomal-exocytosis-of-lipids-protect-neurons) and Ana with her fresh off the press pre-print about Rab nanodomains formation driven by phase partitioning on endosomes https://www.biorxiv.org/content/10.1101/2023.04.17.537227v1
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RT @ishier_raote

https://twitter.com/ishier_raote/status/1648996083287162881

Autolysosomal exocytosis of lipids protect neurons from ferroptosis

Ralhan et al. show that neurons release peroxidated lipids and iron as lipid-rich particles by autolysosomal exocytosis during oxidative stress. This protects n

Rockefeller University Press