Mastodawn

Francesca Bottanelli Feb 21, 2024

MCDuncanLab Feb 21, 2024

PSA for #westernblots

Did you know you can often leave out the methanol and SDS and get an excellent transfer?

Our recipe for 4 liters of 10 x is 560g Glycine, 120g Tris Base.

We transfer at 0.5mA for 30 min for most proteins 40 for >150kDa in a biorad rig.

We use an ice block but no stir bar. It gets hot so don't go longer than 40 min.

#Biochemistry #WesternBlotHelp

Francesca Bottanelli Feb 21, 2024

#MembraneTrafficking seminars are back in 2024! Join Felix Campelo, Ishier Raote and me next week for two exciting talks. Looking forward to seeing all you membrane enthusiasts again!

Feb 29, 5.00pm CET, 11.00am ET

Francesca Bottanelli May 5, 2023

RT @BarnabaCarlo
Here we are again! @DavidGBroadbent @jenscs83 #autophagy @AIM_autophagy @autophagyuk @AutophagyPapers @WAutophagy https://twitter.com/jcellbiol/status/1654520138190692352

JCellBiol on Twitter

“Quantitative analysis of #autophagy reveals the role of ATG9 and ATG2 in autophagosome formation, say David Broadbent @DavidGBroadbent, Carlo Barnaba @BarnabaCarlo, Gloria Perez, & Jens Schmidt @jenscs83 https://t.co/Z5xeW26met”

Twitter

Francesca Bottanelli May 5, 2023

RT @JCellBiol
STED imaging of endogenously tagged ARF #GTPases reveals their distinct nanoscale localizations, say Luis Wong-Dilworth @FreeThePipette, Francesca Bottanelli @fran_bottanelli and colleagues
#microscopy

Francesca Bottanelli Apr 28, 2023

RT @RockUPress
New in @JCellBiol: @FreeThePipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases in living cells https://bit.ly/3HhBHcp

STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations

Wong-Dilworth et al. combine CRISPR-Cas9 gene editing with STED super-resolution microscopy to reveal the specific localization of ARF GTPases on different intr

Rockefeller University Press

Francesca Bottanelli Apr 27, 2023

A tour the force of #geneediting and #STED. We have followed up on some of these findings and have 2 papers that will drop soon! looking at endogenous proteins at high resolution in their own unperturbed cellular environment is helping to reveal important trafficking mechanisms!
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RT @JCellBiol
.@FreeThePipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localizati…
https://twitter.com/JCellBiol/status/1651620746806820872

JCellBiol on Twitter

“.@freethepipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases in living cells https://t.co/2rwNuGu0Iz”

Twitter

Show thread

Francesca Bottanelli Apr 27, 2023

zoom link on here https://felixcampelo.wixsite.com/membranetrafficking @felixmendu @ishier_raote

MEMBRANE TRAFFICKING | Membrane Trafficking

Membrane Trafficking

Francesca Bottanelli Apr 27, 2023

Do not miss today's session with @IoannouLab talking about autolysosomal exocytosis in neurons (https://rupress.org/jcb/article-abstract/222/6/e202207130/214014/Autolysosomal-exocytosis-of-lipids-protect-neurons) and Ana with her fresh off the press pre-print about Rab nanodomains formation driven by phase partitioning on endosomes https://www.biorxiv.org/content/10.1101/2023.04.17.537227v1
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RT @ishier_raote

https://twitter.com/ishier_raote/status/1648996083287162881

Autolysosomal exocytosis of lipids protect neurons from ferroptosis

Ralhan et al. show that neurons release peroxidated lipids and iron as lipid-rich particles by autolysosomal exocytosis during oxidative stress. This protects n

Rockefeller University Press

Francesca Bottanelli Apr 20, 2023

RT @ishier_raote

Francesca Bottanelli Apr 17, 2023

Brooke has been a wonderful mentor to over 40 undergrads in the last 7 years. What a great legacy! We need more people like him to train the next generation of scientists!
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RT @TIRscienceblog
New posting! Another more personal one, about why I've no regrets about staying in #academia as long as I did (& how we should all perhaps be regularly asking ourselves why we're staying).
https://totalinternalreflectionblog.com/2023/04/16/n…
https://twitter.com/TIRscienceblog/status/1647711391359598593

No regrets (a salute to all my students)

Some of the alumni of the Morriswood group. I might be leaving academia, but I’ve no regrets about staying as long as I did.

Total Internal Reflection