BioResireMar 10
Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology
Giuseppe MichieliFeb 24
Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html
Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains

Eli Roberson (he/him)Jan 9

#pcr #qpcr #rtqpcr #academia

Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?

I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.

Teresita Porter 🙋🏻‍♀️Oct 10

"Sexing From Seawater: Application of Environmental DNA Beyond Species Detection for Cetaceans"

#eDNA #environmentalDNA #qPCR

https://onlinelibrary.wiley.com/doi/10.1002/edn3.70173

devSJR  Jun 21, 2025

Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess

- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407>

RoedigerRGApr 25, 2025

Our paper "MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines" is now published in Clinical Chemistry! 📚🔬

This update addresses recent advances in qPCR technology, providing clear recommendations on sample handling, assay design, and data analysis. We emphasize transparency and reproducibility to enhance the reliability of qPCR research.

https://doi.org/10.1093/clinchem/hvaf043 #MIQE2_0 #MIQE #qPCR #ResearchIntegrity #ScientificMethodology 📊🔍

IL PhotonicsNov 14, 2024
Quantitative polymerase chain reaction (#qPCR) is a variation of the traditional PCR methodology to not just rapidly amplify and copy a DNA sample but to count how much has been produced. Real-time qPCR is now considered to be the gold standard method in microarray analysis, which allows for the study of the expression levels of many #genes simultaneously. Learn more: https://ilphotonics.com/what-pcr-filter-challenges-affect-high-performance-qpcr-instruments/
What PCR Filter Challenges Affect High-Performance qPCR Instruments? | IL Photonics

Realtime qPCR in microarray analysis enables the study of the expression levels of many genes simultaneously.

IL Photonics
RoedigerRGOct 24, 2024

For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.

We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats

Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)

#JOSS

OpenAlex

William PerrySep 11, 2024
Great to be involved in this lovely Water Research paper led by the brilliant Maggie Knight at Bangor University! The paper compares the pros and cons of #qPCR vs #metagenomic approaches for detecting antibiotic resistance genes in wastewater. The comprehensive dataset used in the study was from the the widespread Wales wastewater monitoring. Personally, I'm team metagenomic!
http://doi.org/10.1016/j.watres.2024.121989
National-scale antimicrobial resistance surveillance in wastewater: A comparative analysis of HT qPCR and metagenomic approaches

Wastewater serves as an important reservoir of antimicrobial resistance (AMR), and its surveillance can provide insights into population-level trends …

devSJR  Jul 28, 2024

Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us.

#rstats