Moritz NegwerNov 2, 2024

Interesting, though complex, signal amplification strategy using Nanobodies coupled to peroxidase -> added Glucose Peroxidase -> (reaction glucose -> H2O2) -> tyramide-based signal amplification -> fluorophore deposition.

A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
Yamauchi et al., preprint at biorxiv 2024
https://doi.org/10.1101/2024.10.25.620157

#tissueclearing #immunostaining #neuroscience #microscopy

Show threadMoritz NegwerNov 16, 2023

@domhenri Wow, those numbers are depressing.

Figure 3 should scare everyone who uses antibodies (and hopefully spur them to sanity-check their stainings with additional techniques).

https://elifesciences.org/reviewed-preprints/91645v1#fig3

#bioscience #immunostaining #immunofluorescence

Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications