All ScientificalGallstone disease as a cause of abdominal pain #gallstone #abdominalpain #gallstonetreatment ... Continue to: https://www.youtube.com/watch?v=WY0NRvHpR4k
WikiPathwaysInsight into the mechanism of gallstone disease by proteomic and metaproteomic characterization of human bile. https://doi.org/10.3389/fmicb.2023.1276951 #Bile #Metaproteomics #Gallstone #Microbiome #Proteomics
Insight into the mechanism of gallstone disease by proteomic and metaproteomic characterization of human bile
IntroductionCholesterol gallstone disease is a prevalent condition that has a significant economic impact. However, the role of the bile microbiome in its development and the host’s responses to it remain poorly understood.MethodsIn this study, we conducted a comprehensive analysis of microbial and human bile proteins in 40 individuals with either gallstone disease or gallbladder polyps. We employed a combined proteomic and metaproteomic approach, as well as meta-taxonomic analysis, functional pathway enrichment, and Western blot analyses.ResultsOur metaproteomic analysis, utilizing the lowest common ancestor algorithm, identified 158 microbial taxa in the bile samples. We discovered microbial taxa that may contribute to gallstone formation, including β-glucuronidase-producing bacteria such as Streptococcus, Staphylococcus, and Clostridium, as well as those involved in biofilm formation like Helicobacter, Cyanobacteria, Pseudomonas, Escherichia coli, and Clostridium. Furthermore, we identified 2,749 human proteins and 87 microbial proteins with a protein false discovery rate (FDR) of 1% and at least 2 distinct peptides. Among these proteins, we found microbial proteins crucial to biofilm formation, such as QDR3, ompA, ndk, pstS, nanA, pfIB, and dnaK. Notably, QDR3 showed a gradual upregulation from chronic to acute cholesterol gallstone disease when compared to polyp samples. Additionally, we discovered other microbial proteins that enhance bacterial virulence and gallston...
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