As we started by an unbiased screening in the human plasma, we needed to optimize our already published HUNTER method (https://doi.org/10.1074/mcp.TIR119.001560) for high-throughput and the demands of handling a larger cohort with 143 patient samples (in the end), optimizing timing and efficiency. The improvements were heating it up, making it faster and using a stream-lined approach avoiding multiplex dimethyl labeling to enable "label-free-like" N-terminomics. #Ntermini #SHUNTER 2/n

On the #workbench this evening - fitting a Zen decoder to my #Hornby 08 #shunter.

This was the second decoder I tried, after I found the first one was a millimetre or so too thick and prevented the body from sitting properly on the chassis.

Miraculously I managed to:
a) get the body back on
b) not lose any retaining screws
c) not break anything!

#Trains #Modelling #ModelRaikway #ModelRailroading #DCC

The 08 shunter, a workhorse of Britain’s railway depots and yards since the 1950s, may still have a long life ahead of it: https://www.railwaygazette.com/uk/class-08-diesel-shunter-converted-to-battery-power/65199.article

The battery-operated 08e has been built from an ex-Inverness 08 by Positive Traction: https://08-e.positivetraction.co.uk

📷 1. Peter Broster, CC BY 2.0 Deed, via Wikimedia Commons
📷 2. Railway Gazette article linked above

#rail #railway #shunter #shunting #battery

Class 08 diesel shunter converted to battery power

UK: A Class 08 diesel shunting locomotive originally built in the 1950s has been rebuilt to use battery power. Branded 08e, the prototype has been developed by Positive Traction to offer a cost-effective and emission free alternative to diesel power for shunting and industrial applications. 'Class ...

Railway Gazette International
Class 09 024 on the East Lancashire Railway
#Preservedrailways
#elr
#shunter
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On the #workbench this evening - fitting a Zen decoder to my #Hornby 08 #shunter.

This was the second decoder I tried, after I found the first one was a millimetre or so too thick and prevented the body from sitting properly on the chassis.

Miraculously I managed to:
a) get the body back on
b) not lose any retaining screws
c) not break anything!

#Trains #Modelling #ModelRaikway #ModelRailroading #DCC

And the end result was quite smooooooth! 😃👍
Damn! Looks like I didn’t get the body mounted correctly after all! Try again.