Fatourou et al use biological proxies to investigate the connection of the Gulf of Corinth in Greece with the Mediterranean Sea during the Middle and Late Pleistocene. The connection for the past 400,000 years has been through the western Rion Sill, which is currently at 60 m depth.
During glacial periods, sea level fell sufficiently to isolate the Gulf of Corinth from the Mediterranean Sea. Therefore, this record can be used as a way to estimate global ice volume. The major glacial periods of MIS 18, 14, 8, 6, 4 and 2 show a complete isolation of the Gulf.
The major interglacials of MIS 17, 13, 11 and 9 indicate fully connected conditions. MIS 7 shows fluctuations, indicating that ice volume varied. This is perhaps not surprising, since there is a large interruption in MIS 7 with a sharp rise in δ¹⁸O in the marine records, likely a brief glaciation.
During MIS 5, there is also fluctuations, with evidence of isolation during the MIS 5b stadial. During MIS 3, there is evidence that sea level must have risen enough that there was periodically a connection with the Mediterranean Sea (i.e. sea level > -60 m).
Obviously, pin-pointing a specific sea level from these data is difficult, since the height of the sill likely varied due to tectonics, and the "connection" based on these proxies probably does not depend precisely on sea level being above or below -60 m.
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https://doi.org/10.1016/j.quascirev.2025.109393