Rob BeagrieJun 19, 2023
Out today in Nature Methods: our manuscript outlining improvements we've made to Genome Architecture Mapping (GAM) and comparisons between GAM and Hi-C.TLDR: We made GAM much cheaper, show that it performs better than Hi-C at detecting complex interactions and identify a class of CTCF-enhancer interactions that are poorly detected by Hi-C. Paper at https://doi.org/10.1038/s41592-023-01903-1 and the story behind the work at https://protocolsmethods.springernature.com/posts/appreciating-the-complexity-of-dna-folding #genomics #GenomeFolding #epigenetics #paper #ctcf #HiC
Hao YinFeb 25, 2023

#Cohesinopathy

BRD4 (acetyl lysine reader)-NIPBL (cohesin loading ) complex is critical to #NeuralCrest differentiation into #SmoothMuscleCell

BRD4 depletion->⏬#GenomeFolding+loop extrusion

BRD4 in #AorticAneurysm?

Dr. Rajan Jain lab Nature Genet 2021
https://www.nature.com/articles/s41588-021-00934-8

BRD4 orchestrates genome folding to promote neural crest differentiation - Nature Genetics

Depletion of BRD4 reduces the chromatin occupancy of NIPBL, resulting in aberrant genome folding. Loss of BRD4 impedes neural crest differentiation, which can be rescued by depletion of WAPL.

Nature
Rob BeagrieFeb 20, 2023

Re-posting an #introduction after moving server:

My research group at the Wellcome Centre for Human Genetics in Oxford uses #SingleCell techniques and Genome Architecture Mapping to study #epigenetics #Chromatin and #GenomeFolding in rare #NeurodevelopmentalDisorders.

I (He/Him) love to be outside #Hiking or #Cycling and I’m a big fan of #FlightFree travel. Interested in #Equity issues in STEM, trying to read and educate myself to be a better ally.

Toot at me with your #Andor theories!

Rob BeagrieNov 20, 2022

About time for an #introduction. My research group at the Wellcome Centre for Human Genetics in Oxford uses #SingleCell techniques and Genome Architecture Mapping to study #epigenetics #Chromatin and #GenomeFolding in rare #NeurodevelopmentalDisorders.

I (He/Him) love to be outside #Hiking or #Cycling and I’m a big fan of #FlightFree travel. Interested in #Equity issues in STEM, trying to read and educate myself to be a better ally.

Toot at me with your #Andor theories!