Thought-provoking preprint about the variable photostability of StayGold (compared to classic #GFPs) under different imaging modalities. Basically, you should a spinning disk for best results, not pointscanning confocal. Wonder if that also goes for 2-photon. The paper includes a “classification of #fluoresence #microscopy” according to ISO 10934:2020 !!!

https://www.biorxiv.org/content/10.1101/2023.11.23.568490v1.full.pdf