Jonas Wietek 

@[email protected]
338 Followers
280 Following
184 Posts
Postdoc with @oferyizhar at the Weizmann institute of Science. Exploring synaptic inhibition with optogenetics.
GoogleScholarhttps://t.co/sv1yClUUGQ
RGhttps://www.researchgate.net/profile/Jonas-Wietek
orcidhttps://orcid.org/0000-0002-8079-7042
Show threadJonas Wietek May 30, 2024
Here you can find an up-to-date overview of available resources (all plasmids and many AAV variants):
https://docs.google.com/spreadsheets/d/e/2PACX-1vQfQqPasB8NvO3t-OG1CCUwknu9Y_bER34ZsaSgE-peQ4ndwgjIrTPtMpV6lBhigR0XU1tYhwaYrlFz/pubhtml
PdCO resources - Google Drive

Jonas Wietek May 30, 2024

Against all odds: PdCO, a versatile switchable optogenetic tool for inhibiting synaptic transmission in neuronal terminals in vivo is finally published at NMeth

https://www.nature.com/articles/s41592-024-02285-8

A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits - Nature Methods

PdCO is a switchable optogenetic tool for inhibiting synaptic transmission in neuronal terminals in vivo, as demonstrated in a variety of contexts mainly in the mouse.

Nature
Show threadJonas Wietek Jul 2, 2023

Most plasmids soon
@addgene available at http://addgene.org/Ofer_Yizhar/

PdCO-mScarlet: CMV, CaMKII, hSyn, hSyn-SIO, hSyn-DIO, Ef1a-DIO, Ef1a-fDIO
PdCO-EGFP: CMV, CaMKII, hSyn
ChrimsonR-PdCO: CaMKII, hSyn-SIO
... enough to play around with for now😅

Addgene: Ofer Yizhar Lab Materials

Show threadJonas Wietek Jul 2, 2023

No 🧵 (proposal deadline very soon)

PdCO facts:
♡ Genetically encoded
♡ highly efficient
♡ Well expressed
♡ Light sensitive
♡ Precise on- & off-switching
♡ Multiplexable
♡ Signaling via Go (not Gi)
♡ in vivo

Show threadJonas Wietek Jul 2, 2023
https://t.co/ldvIvlfKrs
Jonas Wietek Jul 2, 2023

🚨#Optogenetic preprint alert🚨
Check out PdCO:
Light-controlled inhibition of synaptic transmission via the inhibitory #GPCR pathway. #optogenetics #neuroscience

Link:

Show threadJonas Wietek May 5, 2023
The graphic design is... let's say interesting 😉
Jonas Wietek May 5, 2023
Recommend 👇
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RT @moser_tmoser
Check out Optogenetics 2023 meeting in October near Göttingen, Germany. Great line-up of speakers, exciting topics, nice crowd, great spot!
https://twitter.com/moser_tmoser/status/1654488667065311238
Tobias Moser on Twitter

“Check out Optogenetics 2023 meeting in October near Göttingen, Germany. Great line-up of speakers, exciting topics, nice crowd, great spot!”

Twitter
Jonas Wietek May 5, 2023
🤍💜💙🤎🖤❤️🧡💛💚💙💜
Jonas Wietek May 4, 2023

🤩🤩🤩
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RT @abhi_aggarwal1
iGluSnFR3 is published! Check it out⬇️
https://www.nature.com/articles/s41592-023-01863-6

We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission.

This is #iGluSnFR3 in action detecting minis w/ TTX.
@PodgorskiLab @HHMIJanelia @AllenInstitute
https://twitter.com/abhi_aggarwal1/status/1654141541215084545

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission - Nature Methods

iGluSnFR variants with improved signal-to-noise ratios and targeting to postsynaptic sites have been developed, enabling the analysis of glutamatergic neurotransmission in vivo as illustrated in the mouse visual and somatosensory cortex.

Nature