kevinboldingFrom what I can tell, folks that are successfully using #Neuropixels for either acute or chronic recordings are using the metal cap to attach to a dovetail holder. My question for #neuroscience : is there ever a reason to use the version *without* the metal cap?
The vOICe vision BCI 🧠🇪🇺@kevinbolding When low(er) weight is critical? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177890/
Metal microdrive and head cap system for silicon probe recovery in freely moving rodent
High-yield electrophysiological extracellular recording in freely moving rodents provides a unique window into the temporal dynamics of neural circuits. Recording from unrestrained animals is critical to investigate brain activity during natural behaviors. ...
Chris Rodgers@seeingwithsound @kevinbolding Can I slightly derail this question and ask who is doing freely moving NP in mice? The only paper I have found is Keshavarzi 2022. My impression from talking to people is that NP is too heavy for freely moving mice (except possibly if you select only males and put them through a neck-strengthening exercise routine)
@chrisXrodgers @kevinbolding I'd guess that for freely moving mice the Neuropixels probes are more luggable than wearable, but did you check out https://elifesciences.org/articles/47188 and https://www.cambridgeneurotech.com/pixel-probes? All of this is outside my field of expertise though.
@seeingwithsound great citation, thanks.. can't believe I missed that one.. They come down very reasonably on the issue, "mice are mobile" but it is "quite bulky".
@chrisXrodgers @seeingwithsound the Juavinett is the one I was thinking of. Not sure what else exists but expecting a flood in the near future.
Ben Kanter@kevinbolding @chrisXrodgers @seeingwithsound We do freely moving NP2.0 recordings in mice and rats using custom holders in our lab - movement isn't a big issue for standard foraging tasks. I don't know the exact weight cause I'm on the rat side, but reach out if we can help 👍
Matteo Carandini@chrisXrodgers @seeingwithsound @kevinbolding Hi yes Neuropixels probes can do chronic recordings in freely moving mice, including females, if the implant is light enough. For an overview of available implants see here: https://youtu.be/_M5Uz8KZ6WQ Our lab has developed a particularly light one called "Apollo". It is being used by 20+ labs. Preprint should be on bioRxiv tomorrow or in the next couple of days.
2022 Lecture 06 Chronic recording with Neuropixels (Bimbard and Bauza)
@chrisXrodgers @seeingwithsound @kevinbolding
Here is the Apollo implant: https://www.biorxiv.org/content/10.1101/2023.08.03.551752v1