At 72nd and Broadway
#uws #newyork #nyc
At the corner of 71st and Amsterdam
#uws #nyc #newyork

Just musing with my muse the elusive raven on #MountTom
#UWS
#NYC

A rocky knoll of #Manhattanschist in #RiversidePark overlooks the Hudson River
A few blocks from home, Edgar Allen Poe wrote his poems and stories
I often see a raven there strutting and reciting a speech

#PandaFest yesterday #UWS The delicious aromas and lovely music were so inviting. Delighted to meet a seller who attended the same college in Chengdu where I once taught many decades earlier.
Primate Islet Isolation and Purification in Iodixanol-UWS โ€ข @1MEMO_20260425
by Michel van der Burg, April 25th, 2026

In early 1999, ahead of the official launch of the clinical islet transplantation program at Leiden University Medical Center (LUMC), the author conducted a pilot study to assess whether a novel islet purification method could be applied to non-human primates.

The method combined Iodixanol (Optiprep/Visipaque) with University of Wisconsin Solution (UWS) โ€” a preservation fluid used for donor organ storage. This Iodixanol-UWS approach had proven effective for purifying pig islets since 1996, and had been trialed on human donor islets during a 1998 visit to the Diabetes Research Institute (DRI) in Miami.

Islets were isolated from four non-human primates โ€” one cynomolgus and three rhesus macaques โ€” whose pancreases were procured during euthanasia of animals used in unrelated studies at the Biomedical Primate Research Centre in Holland.

Islets were purified by centrifugation in the Iodixanol-UWS gradient.

Results were excellent: an average yield of 3,697 islets / gram pancreas before purification, with 94% purity and 90% recovery after purification. Viability assessments , and a 98% culture survival confirmed high islet quality.

Colleague Krista Haanstra conducted immunogenicity analyses using a Mixed Lymphocyte Islet Culture (MLIC) assay.

Biosafety protocols for non-human primate tissue proved valuable preparation for the subsequent launch of human islet isolations at LUMC later that month.

Read the full report at michelvanderburg.com/2026/04/25/

#islets #Langerhans #isolation #purification #LUMC #Leiden #primate #macaque #optiprep #iodixanol #UWS #pancreas #transplantation #diabetes #history #research #1memo #michelvanderburg

Citation: Primate Islet Isolation and Purification in Iodixanol-UWS โ€ข @1MEMO_20260425 โ€ข Michel van der Burg โ€ข Miracles.Media โ€ข michelvanderburg.com/2026/04/25/
NYPD on Instagram: "Whether on foot or on hooves, our officers are always ready. After someone stole a purse near West 72nd Street, our Mounted officer & his horse, Kelly, kept on her trail. Galloping down the street & with the help of a witness, they recovered the purse & apprehended the suspect."

31K likes, 572 comments - nypd on April 16, 2026: "Whether on foot or on hooves, our officers are always ready. After someone stole a purse near West 72nd Street, our Mounted officer & his horse, Kelly, kept on her trail. Galloping down the street & with the help of a witness, they recovered the purse & apprehended the suspect.".

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