Show threadRedish LabSep 14, 2025

@susanleemburg @elduvelle

1. Make sure you talk about clustering quality discussions. (In the #tetrode world, papers were required to report L-Ratio and Isolation Distance, which turned out to address type-I and type-II errors in sorting. q.v. https://pubmed.ncbi.nlm.nih.gov/15680687/ )

2. Talk about the fact that spike sorting is an *estimate* and that one needs to think about spikes on the boundary between cluster estimates. (This is why clusterless decoding is so powerful. (Matching decoding to spikes that are nearby in feature space rather than to clustered cells.)

3. Talk about using neuroscience knowledge to judge spike/cluster validity rather than just trusting the system.

Some thoughts off the top of my head.

Quantitative measures of cluster quality for use in extracellular recordings - PubMed

While the use of multi-channel electrodes (stereotrodes and tetrodes) has allowed for the simultaneous recording and identification of many neurons, quantitative measures of the quality of neurons in such recordings are lacking. In multi-channel recordings, each spike waveform is discriminated in a …

PubMed
El Duvelle NeuroNov 14, 2024

#Tetrode recordings (in a bundle): did you know that you could record the same neuron on two different tetrodes?
or even three different tetrodes??

After checking that, turns out I usually have about 5-10% of neurons that are a duplicate of another neuron, in a given 8-tetrodes recording! They are pretty easy to detect with a firing rate correlation so I can remove them from analysis.
But I bet most neuron counts in published papers are inflated of that much!

Here's an example where you can see the spike plots and waveforms of 3 different, well-isolated clusters, recorded from 3 different tetrodes!

#Hexamaze #NeuroRat #Neuroscience #Ephys #Hippocampus #PlaceCells #SpikeSorting (-related)

Tim SchröderApr 1, 2024
Extracellular ephys peeps: Are you building your own microdrives/hyperdrives/implants? In their new preprint on http://bsrxiv.org, van Heument et al systematically analyze how many you should build to make them awesome! Check it out at http://dowhy.org/?127.0.0.1/wtf.bsRx.13 #electrophysiology, doing #openscience using #openhardware in #neuroscience #tetrode #siliconprobes #microdrive @3Dneuro
bsRxiv

El Duvelle NeuroOct 19, 2023

I’ve started making tutorials for different #Tetrode #Electrophysiology steps here
The next ones are probably going to be Tetrode-making and drive-building.

Is there anything else that you’d like to see there?
#NeuroMethods

GitHub - elduvelle/ephys_tutorials

Contribute to elduvelle/ephys_tutorials development by creating an account on GitHub.

GitHub
Show threadEl DuvelleAug 7, 2023
Hmm maybe this one is better… not sure 🤔 😁
#Tetrode #Tetrodes #Hippocampus #Screening
El DuvelleJul 21, 2023

I have to post this…

#Tetrode recordings #Hippocampus #NeuroRat #HybridDrive

Chris RodgersJun 12, 2023
I'm excited to read this #neuroscience #preprint from Keith Hengen's lab: https://www.biorxiv.org/content/10.1101/2023.06.09.544399v1
They use massive arrays of tetrodes spanning the whole brain and record for 24 hours to identify changes in state with sleep/wake. The methods are really cool, in fact Keith taught us to implement them in my own lab for different experiments. The scientific results span experiment and theory and sound really exciting!
#electrophysiology #tetrode #freebehavior
El DuvelleMar 20, 2023
Here are some #PlaceCell clusters for you (#Tetrode recordings) 😃​
#Hippocampus
El DuvelleJan 12, 2023
#Tetrode tips for #Electrophysiology
(17 microns wire Platinum-Iridium)
They are not perfect, but who is? 😉​