Cytology and GeneticsApr 23, 2025
Markers Linked to Stem Rust Resistance Genes Sr39 and Sr40 for Selecting Wheat Breeding Lines - #wheat #Pucciniagraminis #stemrustresistance #raceUg99 #resistancegenes #molecularmarkers #markerassistedbreeding #Puccinia #stemrust #breeding - https://link.springer.com/article/10.3103/S0095452724060069
Markers Linked to Stem Rust Resistance Genes Sr39 and Sr40 for Selecting Wheat Breeding Lines - Cytology and Genetics

Abstract Introduction of genes conferring resistance to Puccinia graminis is considered as the best approach to protect common wheat against stem rust. To facilitate marker-assisted selection of common wheat breeding lines with the stem rust resistance genes Sr39 and Sr40, the testing of molecular markers for these genes was carried out. The markers used for the research were the following: BE500705, Xmag2090, Xmag464, Xcnl158, Xwmc25, Sr39#50, Sr39#22, BCD260, and Xwmc344. Among the simple sequence repeat markers, only Xmag2090, Xwmc25, and Xwmc344 proved to be polymorphic upon analysis of amplicons by polyacrylamide gel electrophoresis followed by silver staining. The markers Sr39#50 and Sr39#22 produced similar amplicons in the control lines RL5711 with Sr39 and RL6089 with Sr40, while amplified fragments were absent in the cultivars. Sr39#50 and Sr39#22 were used for marker-assisted selection of F2 lines from the cross Khutorianka × RL6089 (Sr40) and F4 lines from the cross Solomiia × RL5711 (Sr39). Using Sr39#50, the Sr40 resistance marker was found in 46% of the F2 offspring of the cross Khutorianka × RL6089. Among the F4 offspring of the cross Solomiia x RL5711, the frequency of genotypes with the combination of the Sr39#50 and Sr39#22 marker amplicons was only 11%. Additionally, 33% of the F4 lines showed the Sr39#22 amplicon of approximately 800 bp but lacked the Sr39#50 resistance markers. The reduced frequency of lines with the Sr39 and Sr40 genes may be due to the decreased survival of genotypes with the 2B chromosome introgression after fall planting. The winter wheat lines with the Sr39 or Sr40 gene may be used as the initial material in breeding programs.

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Cytology and GeneticsDec 5, 2023
Development of Winter Common Wheat Lines with the Stem Rust Resistance Gene Sr33 - #wheat #Pucciniagraminis #raceUg99 #stemrustresistance #geneSr33 #markerassistedselection #breedinglines #wintercommonwheat #Puccinia - https://link.springer.com/article/10.3103/S009545272306004X
Development of Winter Common Wheat Lines with the Stem Rust Resistance Gene Sr33 - Cytology and Genetics

Stem rust caused by the fungus Puccinia graminis Pers. is a dangerous disease of wheat that occurs in all regions of its cultivation. New highly virulent races causing severe yield losses have appeared in recent decades. Sr33 introgressed from Aegilops tauschii is one of the genes conferring resistance against most races of stem rust, including Ug99. To develop winter common wheat lines with the gene Sr33 and evaluate a possible effect of the gene on yield traits, the authors made a cross between the spring line DH31 carrying the Sr33 gene and the winter cultivar Myrkhad followed by marker-assisted selection of winter genotypes with Sr33 starting from F3. To identify the Sr33 gene, PCR with the gene-specific marker Sr33A was used. Alleles at the storage protein loci of the parental forms were identified using acid polyacrylamide gel electrophoresis and SDS-electrophoresis as well as the molecular marker MAR for Glu-Blal. As a result of marker-assisted selection, winter F5 lines with the Sr33 gene were developed from the cross DH31 × Myrkhad. The yield traits of F5 spikes of families derived from single F3 spikes with and without Sr33 were analyzed considering that the line DH31 has a specific allele at Gli-D1 from Ae. tauschii, dark glumes, and high molecular weight glutenin subunit alleles associated with high dough strength, in particular, Glu-Blal. Comparison of means of yield traits of spikes from families with Sr33 and without it did not reveal significant differences between these two groups. Thus, winter F5 lines with the Sr33 gene from the cross DH31 × Myrkhad may be used in the breeding practice to develop cultivars with high bread-making qualities of flour and stem rust resistance.

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