#Bioinformatics Q:

I am working on my first #Minion metabarcoding run. I've got a library of dual-indexed amplicons (ITS2). Ran it through the Minion, base called, and now I'm working on demultiplexing.

I have discovered that some of my reads contain multiple amplicons. i.e., a single line in the fastq file includes index1-primer-read1-primer-index2-index3-primer-read2-primer-index4

Is that a problem with my library, the sequencing process, or is it something I can fix at this stage (i.e., computationally)?

Respect to whoever went the extra mile to make this Minion letterbox.

I stopped to photograph it at the entrance to a rural driveway in Lyttelton Harbour. I like how yellow reflectors have been added to it can be seen by car traffic.

Some skilled parent must be very popular with their children. 😀

#letterbox #Minion #DespicableMe #GovernorsBay #LytteltonHarbour #NZ #Christchurch #MetalWork