I asked #biostars : "Parabricks : Number of GPUs requested (2) is more than number of GPUs (0) in the system., exiting."
https://www.biostars.org/p/9614740/

#bioinformatics #parabricks #NVIDIA #haplotypecaller #gatk #gpus

Parabricks : Number of GPUs requested (2) is more than number of GPUs (0) in the system., exiting.

Know what would have been cool? If Broad hadn't broken two thirds of the links in the GATK documentation when they redid the website.

#BroadInstitute #GATK

https://xcancel.com/gatk_dev/status/1908902547521257779 "GATK Dev Team :" GATK forums will be community-driven and self-moderated. They will not be moderated or monitored by a GATK team member. We encourage members of the community to continue to engage with each other on these forums." https://gatk.broadinstitute.org/hc/en-us/community/topics

#gatk

TIL: You can get a list of Spark-enabled GATK tools with the command

gatk --list | grep Spark

(The website doesn't seem to have a list anywhere)

#bioinformatics #GATK #ApacheSpark

Open Source Insights

https://github.com/nf-core/modules/pull/6619 PR accepted for nf-core/modules/gatk/VariantsToTable #gatk 🥳
new module gatk/variants2table by lindenb · Pull Request #6619 · nf-core/modules

This modules implements gatk/variants2table ( was https://nfcore.slack.com/archives/CJRH30T6V/p1725906645564699 ) This comment contains a description of changes (with reason). If you've fixe...

GitHub

So after much community backlash #GATK has reverted back to the ./. representation for no-calls.

The question is, will something like this occur again? and how many people have already switched away to other tools like #bcftools.

I know I have moved all my pipelines back to bcftools and honestly its brilliant.

#genomics #bioinformatics

https://github.com/broadinstitute/gatk/releases/tag/4.6.0.0

Release 4.6.0.0 · broadinstitute/gatk

Download release: gatk-4.6.0.0.zip Docker image: https://hub.docker.com/r/broadinstitute/gatk/ Highlights of the 4.6.0.0 release: We've fixed a serious CRAM writing bug that affects GATK versions...

GitHub

@thatdnaguy You prompted me to properly look it this, so I ran some tests to empirically test when GATK deviated away from #VCF specifications, rather than rely on the unclear changelogs. All the results and datasets are up on my GitHub repo 🧬🧮

https://github.com/lpembleton/gatk4-when-did-dot-leave

TL;DR: missing data representation with ”./.” ceased to exist in #GATK version 4.2.3.0. If you want to retain correct VCF specification and “./.” stick with version 4.2.2.0 or earlier 👍

#genomics #bioinformatics

GitHub - lpembleton/gatk4-when-did-dot-leave: Confirmation of which GATK4 versions go against VCF specifications and call missing GT as 0/0 instead of ./.

Confirmation of which GATK4 versions go against VCF specifications and call missing GT as 0/0 instead of ./. - lpembleton/gatk4-when-did-dot-leave

GitHub
Analytic tool rapidly reveals genetic diversity for next-generation crop breeding

In a major advance for agricultural science, researchers have developed a new computational tool designed to swiftly and efficiently expose genetic diversity within DNA databases of various plant species.

Phys.org