Show threadDavid MasonJul 17, 2025

@roylelab Nice work! This reminds me of a paper published while I was in my PhD lab addressing ER membrane contribution to the Phagocytic cup.

It still stands as a fantastic example of structured hypothesis testing.

https://doi.org/10.1016/j.cell.2005.08.018

#EndoplasmicReticulum #Phagocytosis #macrophages #ScienceMastodon

Jonathan TownsonMar 3, 2025

Very happy to see the review I wrote on mechanosensing by the endoplasmic reticulum (ER) published @J_Cell_Sci! I’ve really enjoyed studying the ER and hope to contribute some research articles soon.

#EndoplasmicReticulum #review #mechanosensing

https://journals.biologists.com/jcs/article/138/4/JCS263503/367029/The-emerging-roles-of-the-endoplasmic-reticulum-in

Jonathan TownsonOct 25, 2024

I really enjoyed the EMBO ER conference. A very friendly bunch who gave lots of useful feedback on my ideas for my postdoc project. Hopefully some will bear fruition and maybe as collaborations. (1/2)

#ER #EMBO #CellularHomeostasis #conference #Biology #EndoplasmicReticulum

Jonathan TownsonOct 20, 2024
Excited to be in Tuscany for the #embo ER cellular homeostasis meeting! Read a lot about the #ER recently but judging from the abstracts there is still a lot left to read/learn! #conference #EndoplasmicReticulum #Italy
preLightsOct 15, 2024

Time to ‘LaBeRl’ your contacts 🏷️

@JontyTownson highlights a #preprint from Laura Downie, @steveroyle and team @roylelab on a new method to label ER-membrane contact sites without changing the number, extent, or distance of the contacts.

Check it out 👀

https://prelights.biologists.com/highlights/non-disruptive-inducible-labeling-of-er-membrane-contact-sites-using-the-lamin-b-receptor-v2/

#preprint #CellBiology #EndoplasmicReticulum #biology

Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor - preLights

Time to “LaBeRl” your contacts: The Royle lab develop a method for labelling ER membrane contact sites without changing the number, extent, or distance of the contacts.

preLights
micrordtMay 1, 2024

Exploring the Endoplasmic Reticulum: A Key Player in Eukaryotic Cells

#Detoxification, #DrugMetabolism, #EndoplasmicReticulum, #ER, #EukaryoticCell, #LipidMetabolism, #Protein, #ProteinSynthesis, #RER, #SER #CellBiology In the intricate world of eukaryotic cells, where life's essential processes unfold with remarkable precision, the endoplasmic reticulum (ER) stands as a vital architectural marvel. This membranous network…. Medical Microbiology & Recombinant DNA…

https://micrordt.wordpress.com/2024/05/01/exploring-the-endoplasmic-reticulum-a-key-player-in-eukaryotic-cells/

Exploring the Endoplasmic Reticulum: A Key Player in Eukaryotic Cells

#Detoxification, #DrugMetabolism, #EndoplasmicReticulum, #ER, #EukaryoticCell, #LipidMetabolism, #Protein, #ProteinSynthesis, #RER, #SER #CellBiology In the intricate world of eukaryotic cells, whe…

Medical Microbiology & RDT Labs
PLOS BiologyNov 9, 2023
Selective macroautophagy of the #EndoplasmicReticulum (ER) and the #nucleus, known as ER-phagy and nucleophagy respectively, are poorly understood. A study in #FissionYeast reveals that conserved ER-shaping protein Yep1 (REEP1-4 ortholog) is required for these processes; in its absence, #ERphagy/#nucleophagy cargo structures accumulate in the cytoplasm #PLOSBiology https://plos.io/464ZfuI
The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast

Selective macroautophagy of the endoplasmic reticulum (ER) and the nucleus, known as ER-phagy and nucleophagy respectively, are poorly understood. This study in fission yeast reveals that a conserved ER-shaping protein is required for these processes; in its absence, ER-phagy/nucleophagy cargo structures (not enclosed within autophagosomes) accumulate in the cytoplasm.

Stephen RoyleNov 4, 2023

Some great tips for ER fixation here:

https://focalplane.biologists.com/2023/11/02/community-collective-imaging-the-secretory-pathway/

Our experience is also that ER fixation can be tricky. One glutaraldehyde-free method that we have used is fixing for 2 h in 2% paraformaldehyde in 87.5 mM lysine, 87.5 mM sodium phosphate, pH 7.4, and 10 mM sodium periodate.

#EndoplasmicReticulum #CellBiology #Microscopy #MembraneTrafficking

Community collective: imaging the secretory pathway - FocalPlane

Community collective: imaging the secretory pathway - Discussions

FocalPlane
Journal of Experimental BotanySep 16, 2023

'#Auxin transport at the endoplasmic reticulum: roles and structural similarity of #PIN-FORMED and PIN-LIKES'

https://doi.org/10.1093/jxb/erad192

#ER #ProtonMotiveForce #Topology #Uniport #EndoplasmicReticulum

Auxin transport at the endoplasmic reticulum: roles and structural similarity of PIN-FORMED and PIN-LIKES

This review discusses auxin transport across the endoplasmic reticulum membrane by non-canonical PIN-FORMED (PIN) proteins and the related PIN-LIKES (PILS) prot

OUP Academic
ℵ₀ 🏳️‍⚧️🏴‍☠️Aug 18, 2023

https://www.sciencedirect.com/science/article/abs/pii/S0898656823002553

The role of #PERK-#eIF2α-#ATF4-#CHOP #pathway in #sevoflurane induced #neuroapoptosis and #cognitivedysfunction in aged mice

10.1016/j.cellsig.2023.110841

#POCD #hippocampus #apoptosis #endoplasmicreticulum #volatileanesthesia #inhalationanesthesia #anesthesia #fluorinatedethers #alzheimers