Took a while, but finally out:

Pruning the Tree: Comparing #OTUs and #ASVs in High-Throughput Sequencing of 5S-IGS Nuclear Ribosomal DNA in #Phylogenetic Studies

How to reduced 100ks of reads down to the 100 really needed, a comparison between #Mothur and #dada2 pipelines using our nigh-#reticulate #beech dataset as test subject

https://doi.org/10.1002/ece3.72242

Just to inform you that a new version of diat.barcode (gathering more than 1000 new rbcl sequences from NCBI). We also added/curated bio and ecological traits for many taxa.
https://doi.org/10.15454/TOMBYZ
version v12.4 here:
https://entrepot.recherche.data.gouv.fr/file.xhtml?persistentId=doi:10.57745/YGS78N&version=16.0
Ready to use database for DADA2:
https://doi.org/10.57745/XWJJGI

PS : in case you find a bug, please tell me !
#metabarcoding #diatom #eDna #omics #nature #algae #microalgae #pollution #DADA2

Intragenomic diversity of the V9 hypervariable domain in eukaryotes has little effect on metabarcoding

https://www.cell.com/iscience/pdf/S2589-0042(23)01368-8.pdf

#18S #v9 #IntragenomicVariation #DADA2 #SWARM #metabarcoding