During this stage I often get help from two additional sources of information:
1. The model from #model_angelo sometimes helps moving the #AF2 model to the correct location, in these cases where a segment of the AF2 model doesn't match the map. Very often these discrepancies between map and prediction point to regions of the protein involved in conformational change, so I make a note of the chain ID and residue range so I know to take a close look later and compare to related structures.
10/19
Using #ChimeraX, I dock these #AF2 models into the map. I have seen a case with two different protein subunits of very similar sequences, which makes assigning each AF2 model to the density pretty difficult. It is however very easy to align (with the `matchmaker` command in ChimeraX) the AF2 models to the model produced by #model_angelo. This will unambiguously place similar AF2 models to where model_angelo detected their sequences.
5/19