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RNA-triggered cell killing with CRISPR-Cas12a2

최근 발견된 CRISPR-Cas12a2는 특정 RNA 인식 시 무차별적인 이중가닥 DNA 절단 활성을 발휘하여 효모와 인간 세포에서 목표 RNA를 발현하는 세포를 선택적으로 제거할 수 있다. Cas12a2는 표적 전사체에 의해 활성화되며, 오프타겟 활성화 없이 세포 사멸을 유도한다. 이를 활용해 인유두종바이러스 감염 세포, 유전자 편집 실패 세포, KRAS의 종양 유발 점 돌연변이 세포를 선택적으로 제거하는 데 성공했다. 기존 Cas9, Cas12a, Cas13 기반 방법들이 진핵세포에서 세포 사멸을 효과적으로 유도하지 못한 한계를 극복하는 새로운 CRISPR 도구로 주목된다.

https://www.nature.com/articles/s41586-026-10466-y

#crispr #cas12a2 #cellkilling #rnatriggered #geneediting

RNA-triggered cell killing with CRISPR–Cas12a2 - Nature

Cas12a2 enables RNA-triggered, sequence-specific killing of eukaryotic cells via widespread DNA shredding, allowing selective elimination of cells on the basis of gene expression, including virus-infected or mutation-bearing cells.

Nature

Helmholtz Institute Würzburg Mar 28, 2023

Check out this CRISPR Medicine article highlighting the recent discovery of the #nuclease #Cas12a2, a new type of #CRISPR gene scissors. ✂️🧬
https://crisprmedicinenews.com/news/appetite-for-destruction-the-indiscriminate-nuclease-activity-of-cas12a2/

News: Appetite for Destruction: The Indiscriminate Nuclease Activity of Cas12a2 - CRISPR Medicine

Researchers from Utah State University and Germany’s Helmholtz Institute for RNA-based Infection Research demonstrate that upon recognition of an RNA target, Cas12a2 cleaves all the other nucleic acids present, destroying the bacterial cell and preventing bacteriophage from replicating.

CRISPR Medicine

Zack Shure Jan 22, 2023

„a new #CRISPR protein — #Cas12a2 — which they say is more like a genetic Swiss Army Knife than a pair of scissors“

https://www.freethink.com/science/crispr-cas12a2

New killer CRISPR system is unlike any scientists have seen

CRISPR-Cas12a2 — a system that causes cells to self-destruct — could lead to new cancer treatments, better diagnostic tests, and more.

Freethink Media