Emergent biosynthetic pathway to essential amino acids by metabolic metathesis
https://www.biorxiv.org/content/10.1101/2023.09.06.556532v1?rss=1
* alkene metathesis unknown in extant biochemistry
* alkenic amino acid vinylglycine can be generated in biocompatible reaction
* vinylglycine produced in situ then used as precursor of isoleucine & methionine
* allows growth of Escherichia coli strains normally requiring these essential amino acids
#biochemistry #MolecularBiology #SyntheticBiology #DirectedEvolution #auxotrophy #prototrophy
.@RalserLab identifies a new H2S utilizing enzyme, Hsu1p, that recycles hydrosulfide & helps growth under
#sulfur limitation, allowing
#yeast to overcome Met17
#auxotrophy when grown in high density, revealing cell-cell metabolic interactions
#PLOSBiology https://plos.io/3B2vWvY
Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy
In yeast, Met17p deletion induces the overflow of hydrosulfide ions due to methionine auxotrophy. This study identifies a new H2S utilizing enzyme, Hsu1p, that recycles hydrosulfide and confers a growth advantage under sulfur limitation; Hsu1p allows the cells to overcome methionine auxotrophy when grown in high density, underscoring the importance of cell-cell metabolic interactions.
.@RalserLab identifies a new H2S utilizing enzyme, Hsu1p, that recycles hydrosulfide & helps growth under
#sulfur limitation, allowing
#yeast to overcome Met17
#auxotrophy when grown in high density, revealing cell-cell metabolic interactions
#PLOSBiology https://plos.io/3B2vWvYInorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy
In yeast, Met17p deletion induces the overflow of hydrosulfide ions due to methionine auxotrophy. This study identifies a new H2S utilizing enzyme, Hsu1p, that recycles hydrosulfide and confers a growth advantage under sulfur limitation; Hsu1p allows the cells to overcome methionine auxotrophy when grown in high density, underscoring the importance of cell-cell metabolic interactions.
.@RalserLab identifies a new H2S utilizing enzyme, Hsu1p, that recycles hydrosulfide & helps growth under
#sulfur limitation, allowing
#yeast to overcome Met17
#auxotrophy when grown in high density, revealing cell-cell metabolic interactions
#PLOSBiology https://plos.io/3B2vWvYInorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy
In yeast, Met17p deletion induces the overflow of hydrosulfide ions due to methionine auxotrophy. This study identifies a new H2S utilizing enzyme, Hsu1p, that recycles hydrosulfide and confers a growth advantage under sulfur limitation; Hsu1p allows the cells to overcome methionine auxotrophy when grown in high density, underscoring the importance of cell-cell metabolic interactions.
Victoria Stuart 🇨🇦 🏳️⚧️
PLOS Biology