27] Unusual Observations about Microbial, Plant, and Animal Polysaccharides

A scalable PDMS extraction method for profiling fungal volatile compounds - Fungal Biology and Biotechnology

Background Fungal volatile organic compounds (FVOCs) play key roles in fungal ecology, physiology, and biotechnological applications, but inconsistent sampling and analytical methods limit biological interpretation and cross-study comparability, underscoring the need for a standardized, validated workflow. Results We developed and validated a polydimethylsiloxane (PDMS)–based volatilomics workflow and evaluated its performance across key methodological dimensions, including solvent extraction bias, static versus dynamic sampling, sorbent reuse, temporal emission resolution, and discrimination of physiological states. Solvent choice (dichloromethane vs. diethyl ether) influenced the quantitative recovery of individual compounds but did not affect the overall FVOC composition detected. Static PDMS and dynamic push–pull sampling produced distinct yet complementary volatilome profiles, with method-specific enrichment across compound classes. Reconditioned PDMS tubing performed equivalently to fresh tubing across repeated deployments, with no detectable decline in compound recovery and multivariate structure. Sequential 96-h sampling captured clear temporal emission patterns in both Trichoderma atroviride and Grosmannia clavigera, revealing species-specific emission trajectories consistent with metabolic stages. Application of the optimized workflow further distinguished T. atroviride morphotypes (white vs. green), which maintained distinct volatile profiles over time and exhibited morphotype-specific emission dynamics in key compounds. Conclusions The PDMS-based workflow presented here provides a robust and reproducible framework for FVOC analysis, effectively addressing methodological biases, resolving temporal emission dynamics, and discriminating among physiological states. Standardizing PDMS sampling and extraction substantially enhance the biological interpretability and comparability of FVOC data, enabling broader and more reliable applications in fungal ecology, physiology, and biotechnology.

Remote Chemistry Expert (PhD)

Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

The Biochemical Beauty of Retatrutide: How GLP-1s Actually Work

On some level, calories in calories out has to be true. But these variables are not independent. Bodies respond to exercise by getting hungry and to calorie deficit by getting tired. Even absent th…

The Biochemical Beauty of Retatrutide: How GLP-1s Actually Work

On some level, calories in calories out has to be true. But these variables are not independent. Bodies respond to exercise by getting hungry and to calorie deficit by getting tired. Even absent th…

A laboratory-designed molecule inspired by nature offers a promising alternative for coeliac disease

Discover celiacase, a newly engineered enzyme that breaks down toxic gluten peptides in the stomach to prevent celiac disease autoimmune reactions.

Remote Chemistry Expert (PhD)

Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

Distinguished Professor in Biology

Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

New method sharpens the search for alien biology

A new statistical method detects extraterrestrial life by analyzing molecular organization and distribution instead of individual chemicals.