Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer

Mutations in mitochondrial DNA (mtDNA) cause a variety of serious multi-organ human diseases, but existing means of preventing mtDNA transmission are problematic. This study presents a novel technically optimized strategy to perform spindle-chromosomal complex transfer (SCCT) with minimal mtDNA carryover and excellent embryo development in both mouse and human models.