Just dropped $1200 to upgrade one of my servers to 512 GB mem. How’s your day?

#teamtomo #cryoet

New Title Alert: Follow_Relion_Gracefully- a complete dashboard for easy interaction with your cryo-EM data in Relion, now with full #teamtomo support!

Learn more here: https://github.com/dzyla/Follow_Relion_gracefully

#SBGridsoftware #StructuralBiology

One highlight from Friday that I forgot: Alister Burt's talk on his efforts to build #TeamTomo, a set of community-supported software packages and metadata standards for #cryoET. This is difficult work, and less rewarding for those in academia, but so essential. It's great to see progress on this front.

The other great news from Alister's talk: Warp is now supported on Linux!

Finally, I'll remember his advice "think about geometry, and use it if you can" when assigning initial particle orientations for subtomogram averaging.

#CCPEM #CryoEM

Okay, so I'm not switching completely to #teamtomo Helical reconstruction of #amyloids can be quite fun when you get beautiful structures like this! And thanks to @sjorsscheres.bsky.social for the suggestion of higher order symmetries, it was indeed the case 😉🧪🧬🧶
Bluesky

Bluesky Social

#TeamTomo is having a competition to annotate #cryoET tomograms: https://cryoetdataportal.czscience.com/competition

This is a good initiative! Especially the metadata format standardization part. I hope several good 3D particle picking programs will emerge from this.

#StructuralBiology

https://www.biorxiv.org/content/10.1101/2024.05.30.596601v1

We made Surforama to support particle picking on surfaces, particularly for cryoET. You can visualize surfaces in the tomogram to localize, label, and analyze proteins or other particle types.

It runs as a @napari plugin and in standalone mode.

#teamtomo

Come see our deeper dive into human chromatin in situ at #cellbio2023. #teamtomo study led by Jon Chen and Tingsheng Liu. Poster P2005, Board B281, Monday Dec 4. Preprint: https://www.biorxiv.org/content/10.1101/2023.07.31.551204v1.full
5/5 A big thank you to our collaborators and #teamtomo!
Very excited to share our paper. https://www.biorxiv.org/content/10.1101/2023.10.26.564098v1
We combine cutting-edge developments in cryo-electron tomography with hiPSC-cardiomyocytes to visualize cardiac proteins in their cellular context in health and disease. #cryoem #teamtomo #cryoet
🧵 1/5

Cool article about the current state of #cryoET #teamTOMO

Catching proteins at play: the method revealing the cell’s inner mysteries
https://www.nature.com/articles/d41586-023-02909-7

Catching proteins at play: the method revealing the cell’s inner mysteries

Cryo-electron tomography is a hugely promising tool in visual proteomics — if researchers can work out what they are seeing.