New cytosine base editors!
https://www.nature.com/articles/s41587-022-01532-7
https://www.nature.com/articles/s41587-022-01533-6
(Another by Nicole Gaudelli's team apparently coming soon.)
Why care:
Base editors can install predictable gene edits without DNA breaks. The described C-to-T editors are as efficient at current tools, but create less off-target damage in the genome and are smaller, which helps with delivery in vivo.
#CRISPRpapers hashtag?
Let's start with detection of large CRISPR edits.
https://www.science.org/doi/10.1126/sciadv.abo7676
Why care:
Often first questions after #CRISPR editing is: What mutations do I have? Unfortunately, that can be hard to answer. Target sites are typically PCR amplified and sequenced, but that has a strong survivor bias. PCR favors some sequences, creates chimeras and fails if primer site is deleted. This paper presents a workflow to mitigate some of these issues and get a more complete picture.
Fillip Port