Witold Szymański

@[email protected]
98 Followers
104 Following
199 Posts
Profile administered by Witold Szymański.
I am a staff scientist responsible for Mass Spectrometry part of our Institute for Translational Proteomics and the Core Facility for Proteomics at the Phillips University of Marburg, Germany.
websitehttps://www.graumannlab.science/
Witold SzymańskiSep 21, 2023
Let's try something new as well. Anyone interested, please follow me on Bluesky:
@proteomicsmarburg.bsky.social
Show threadWitold SzymańskiSep 21, 2023
@pastelbio You have some invite codes @neely ? :)
Witold SzymańskiSep 20, 2023
Show threadFatih DemirSep 20, 2023
@translational_proteomics @UCDProteomics You can do it in 1x PBS or 4% SDS/HEPES buffer, I prefer second.
Show threadWitold SzymańskiSep 19, 2023
@pwilmart @neely @UCDProteomics don't forget the pie charts and some 5 directional, scaled Venn diagrams.
Show threadWitold SzymańskiSep 19, 2023
@pwilmart @UCDProteomics
Show threadWitold SzymańskiSep 19, 2023
@kabalak @UCDProteomics 😅 it was just about the CAM abbreviation! I was quite sure, you're talking about some special depletion method I don't know 😏 what do you dilute the plasma with?
Show threadWitold SzymańskiSep 19, 2023
@kabalak @UCDProteomics Ok, I need help here. Probably a problem with abbreviations, but I have no idea what you mean by CAM process. :D I searched the whole literature in order not to look like a moron, but I didn't find anything. The only thing that comes to my mind is Carbamidomethylation, but then, I don't understand how you do it directly on plasma.
Show threadWitold SzymańskiSep 19, 2023
@UCDProteomics @pwilmart you can then spend 400k years, trying to deconvolute the signal from a particular single cell in your sample.
Witold SzymańskiSep 19, 2023
Show threadFatih DemirSep 18, 2023
@translational_proteomics @UCDProteomics Prepping plasma? I thought it is always ready to use ;-) Normally, we just dilute strongly (1:10 for human) and start the CAM process, followed by SP3 cleanup .
Show threadWitold SzymańskiSep 15, 2023
@pastelbio a link please?