Mastodawn

Witold Szymański Sep 21, 2023

Let's try something new as well. Anyone interested, please follow me on Bluesky:
@proteomicsmarburg.bsky.social

Witold Szymański Sep 20, 2023

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Fatih Demir Sep 20, 2023

@translational_proteomics @UCDProteomics You can do it in 1x PBS or 4% SDS/HEPES buffer, I prefer second.

Witold Szymański Sep 19, 2023

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Fatih Demir Sep 18, 2023

@translational_proteomics @UCDProteomics Prepping plasma? I thought it is always ready to use ;-) Normally, we just dilute strongly (1:10 for human) and start the CAM process, followed by SP3 cleanup .

Witold Szymański Sep 13, 2023

Found an old spectrum from 2011! :D

Witold Szymański Sep 2, 2023

Phil Wilmarth Sep 2, 2023

Most failed proteomics methods are things that sounded good on paper but something was fundamentally wrong or the technology was not capable. I have no idea how random enrichment of protein noise from plasma ever got off the ground. It sounds ridiculous on paper. Let's see if some nanoparticles with different surfaces can have a really rare peptide stick before the crap from albumin saturates everything. Maybe there are non-random patterns in the random binding. Time to go commercial…

Witold Szymański Aug 28, 2023

New paper published with the involvement of our lab!

https://pubmed.ncbi.nlm.nih.gov/37607954/

Impaired Plakophilin-2 in obesity breaks cell cycle dynamics to breed adipocyte senescence - PubMed

Plakophilin-2 (PKP2) is a key component of desmosomes, which, when defective, is known to promote the fibro-fatty infiltration of heart muscle. Less attention has been given to its role in adipose tissue. We report here that levels of PKP2 steadily increase during fat cell differentiation, and are c …

PubMed

Witold Szymański Aug 26, 2023

Ohh how I missed that! Gravel ride!

Witold Szymański Aug 15, 2023

PastelBio Aug 15, 2023

RT @DemichevLab: We have added DIA-NN 1.8.2 beta 25 to the QuantUMS preprint repository https://osf.io/q8kfc/?view_only=5e77d3c62563468280fd09265583dbbd. Inlcudes improved QuantUMS and some fixes. This version has quite a number of major improvements over 1.8.1, we will make an official github release once thoroughly tested https://osf.io/q8kfc/?view_only=5e77d3c62563468280fd09265583dbbd

QuantUMS benchmarks

Benchmarks of the QuantUMS algorithm for peptide and protein quantification from LC-MS proteomics data Hosted on the Open Science Framework

OSF

Witold Szymański Aug 4, 2023

K K Murray (RSS)Aug 3, 2023

(RSS) (Proteom Res News) Confirmatory results! 0.1% formic acid IS a stupid buffer, particularly for single cells!: A few months ago, Battellino et al., dropped this bombshell little paper on the proteomics world that made me get up and start questioning everything. Their title was long but I called it "is using formic acid in your buffers stupid?" and that sort of summed it up.

Caution! Disclaimers! Check with the persons who service… https://proteomicsnews.blogspot.com/2023/08/confirmatory-results-01-formic-acid-is.html?utm_source=dlvr.it&utm_medium=mastodon (/Proteom Res News) #proteomics

Confirmatory results! 0.1% formic acid IS a stupid buffer, particularly for single cells!

A few months ago, Battellino et al., dropped this bombshell little paper on the proteomics world that made me get up and start questioning e...

Witold Szymański Aug 3, 2023

PastelBio Aug 3, 2023

@proteomicsnews blog post | Confirmatory results! 0.1% formic acid IS a stupid buffer, particularly for single cells! | https://proteomicsnews.blogspot.com/2023/08/confirmatory-results-01-formic-acid-is.html #proteomics

Confirmatory results! 0.1% formic acid IS a stupid buffer, particularly for single cells!

A few months ago, Battellino et al., dropped this bombshell little paper on the proteomics world that made me get up and start questioning e...