Fantastic #cryoem #cryoet Rotavirus study from @proteincapsid in the Mark Boyce and David Stuart labs @OxfordStrubi. Truly amazing to see the potential of in situ methods to address the dynamic nature of proteins and reveal previously unseen conformations. https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(23)00108-7
Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography
Shah et al. used cryo-tomography to characterize the assembly pathway of rotavirus inside infected cells. The use of flash frozen samples preserved the assembly stages in their native state and relative proportions, and subtomogram averaging revealed their molecular structures, to near atomic detail in the best case.




