Navin B Ramakrishna

@[email protected]
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Research Fellow, Jay Shin Lab, Genome Institute of Singapore. Former Reversade Lab :: PhD Miska & Surani Labs, Gurdon Institute, Cambridge :: Biochem Grad, Oxford. Twitter: @navinbrian
Navin B RamakrishnaJan 7, 2023

An interesting investigation into the periods of human germline competence across the pluripotency spectrum.
Depending on origin, hPGCLCs show progression differences in hindgut co-culture. Glad to have contributed a little! Led by João & Freddy.

Link: https://cell.com/cell-reports/fulltext/S2211-1247(22)01806-X

Show threadNavin B RamakrishnaDec 5, 2022

So, will we have a mammalian in vitro fetal piRNA model?
Ishikura et al 2021’s reaggregated rTestis m5/7 cells and gonocyte-like cells (GSCLCs) look v. promising, with marked fetal piRNA pathway upregulation.

https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00342-8?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1934590921003428%3Fshowall%3Dtrue.

Show threadNavin B RamakrishnaDec 5, 2022
By expression analysis of the differentiation from mESCs, we do not detect robust expression of the piRNA pathway in D6 mPGCLCs.
Crucially, from small RNA-seq of 3 more lines (different strain backgrounds) including the E14 line, we do not detect piRNAs.
Navin B RamakrishnaDec 5, 2022
Glad and relieved to share on mastodon my first co-first work from one of my PhD projects with Eric Miska & Azim Surani. ☺️ Hope it’s a useful one: we team with Giorgia Battsitoni and Greg Hannon to investigate a prior observation of piRNAs in mPGCs in vitro.
Alas, we do not detect piRNAs in Day 6 mPGCLCs. https://www.cell.com/developmental-cell/fulltext/S1534-5807(22)00783-3
#piRNAs #GermCells #mPGC #mPGCLC #DevCell #MattersArising