Great new addition to the #preLights5thBirthday #preList!
Samantha Seah, Navin B Ramakrishna & Hui Ting Zhang #astar_gis highlight a #preprint from the #FergusonSmith Lab.
Probabilistic inheritance of intermediate DNA #methylation sites across somatic cell division
An interesting investigation into the periods of human germline competence across the pluripotency spectrum.
Depending on origin, hPGCLCs show progression differences in hindgut co-culture. Glad to have contributed a little! Led by João & Freddy.
Link: https://cell.com/cell-reports/fulltext/S2211-1247(22)01806-X
Hey everyone, Happy Holidays! I come bearing the gift of our last preprint of 2022.
https://www.biorxiv.org/content/10.1101/2022.12.26.521943v1
Will probably run a legit "Toot-orial" in the New Year when people are back, but briefly: We have long been interested in how the genome is packaged in sperm, both as a cool chromatin state and also because several labs have reported that mutations that affect histone modifications in the testis have intergenerational effects. 1/n
It appears that PGCLCs stall in development likely due to an incorrect physical & signalling environment in EBs.
See Cooke & Moris (2021) for an excellent discussion on the mechanics and extra-cellular cues of this differentiation
Summary: This Review summarises recent research revealing that primordial germ cell (PGC) development depends on interactions with the surrounding somatic cells and tissues, and that this co-development is crucial for PGC specification, migration and maturation.
So, will we have a mammalian in vitro fetal piRNA model?
Ishikura et al 2021’s reaggregated rTestis m5/7 cells and gonocyte-like cells (GSCLCs) look v. promising, with marked fetal piRNA pathway upregulation.
Although this was a protracted journey since 2017, I’m very grateful that, Wolf Reik’s lab relooked at the original data in von Meyenn et al 2016 and graciously corrected a mistake with their small RNA data.
Lots went on behind the scenes – but this is an instance of science working well! https://www.cell.com/developmental-cell/fulltext/S1534-5807(22)00809-7
preLights
The EMBO Journal
Ollie Rando