9/ 🔍 We invite clinicians, surveillance labs & researchers to explore the dataset & share your feedback.

Feedback? Questions? Drop a comment, DM or on GitHub. Like to join us in creating more EV datasets? Please reach out!

Thank you to everyone working on #Enteroviruses!🙏🏼

8/ 🙏🏻With our thanks:

This dataset was created by Nadia Neuner-Jehle (@n9j), Alejandra Gonzalez Sanchez, & Emma Hodcroft (@firefoxx66) - with help from Nextclade creators Richard Neher (@richardneher) & Ivan Aksamentov - and the input and advice of the ENPEN community!

7/ 🔜 Looking ahead:

We’re planning expansions - other non‐polio enteroviruses such as EV-A71, CVA10, CVA16.

🔭 Future features we hope to add: a recombination QC flag, gene-specific clade assignments.

Stay tuned & we welcome your input!

6/ 💻 Running locally:

You can also use the new EV-D68 dataset locally via the Nextclade CLI - perfect for integrating into workflows and sequencing pipelines!

Find out more about the CLI here: https://docs.nextstrain.org/projects/nextclade/en/stable/user/nextclade-cli/index.html

5/ 💡 How to use it (cont):

- In the results: you’ll see QC status (red/yellow/white), clade calls, mutation counts, coverage gaps, etc. 🔠🚩💯

- The tree view places your sequences into the global phylogeny alongside curated reference data.

4/ 💡 How to use it:

- Go to nextclade.org → choose the EV-D68 dataset or go to https://clades.nextstrain.org/?dataset-name=enpen/enterovirus/ev-d68

- Drag & drop your FASTA (or load example)

- Click Run - alignment, QC & clade assignment happen in your browser (no data leaves your computer)

3/ What the D68 dataset can do:

- Clade assignments (A1–A2/D, B1–B3, C) 🔠

- Whole-genome mutation calling (not just VP1) Ⓜ️

- Quality-control (QC) metrics 🚩

- Gene annotations across the genome 🧬

👉🏻 So even if VP1 is missing, you can still assign the clade reliably.

2/ Why this is important:

📈 EV-D68 cases are already rising this season.

📂 This dataset lets clinicians & researchers quickly run clade assignments, mutation calls, and QC checks directly in their browser - with no data uploaded

Detailed info here!: https://eve-lab.org/blog/EV-D68-on-Nextclade/