Dr. Jon Puritz

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Assistant professor at the University of Rhode Island. Interested in human impacts on the evolution of marine populations. #NewPI [he, his, him]

Checkout the lab account @MarineEvoEcoLab

Websitehttps://MarineEvoEco.com
Githubhttps://github.com/jpuritz
Google Scholarhttps://scholar.google.com/citations?user=l3CNKC4AAAAJ&hl=en&oi=ao
ORCIDhttps://orcid.org/0000-0003-1404-4680

I didn’t know until now, but I was born to decline faculty meeting calendar invites.

#FirstSabbatical

For those of you doing TRIS calibration for #CarbonateChemistry, what temperature probe do you use? We've been using this: https://fishersci.com/shop/products/traceable-platinum-ultra-accurate-digital-thermometer/15081102 but it breaks yearly.
Fisherbrand Traceable Platinum Ultra-Accurate Digital Thermometer - Thermometers and Temperature Measurement, Digital Thermometers

High-precision unit is designed for years of reliable service—even in the severest environments.

What a semester! | Puritz Lab of Marine Evolutionary Ecology

As I sit and reflect on the Spring 2024 semester, I am just amazed at all the incredible achievements of the PLOMEE team! We started the Spring semester with Gabe Barret defending his Master’s thesis, “Variant Graphs Improve Accuracy of Downstream Analysis In Restriction Site-Associated DNA Sequencing.

Puritz Lab of Marine Evolutionary Ecology
@ELSimms I agree. The exchange just immediately made me think of that Far Side comic. Also why I was being cheeky saying that it could be a good sign!
WARNING: if you’re using a colored and shadowed font and a 4:3 format in PowerPoint, I’m immediately questioning how many decades old the presentation is.

Out now! A dynamic web resource for robust and #reproducible #genomics in nonmodel species
https://marineomics.github.io/

This paper in @MethodsEcolEvol describes our web resource for genomics pipelines. Examples include functional genomics, population genomics #PopGen, and genome-phenome. More pages are coming soon.
Many examples use #rstats and datasets for marine organisms. Consider contributing to the site! It's a great way to achieve broader impacts for your work.

Welcome

JUST PUBLISHED: "A second unveiling: Haplotig masking of the eastern oyster genome improves population-level inference" #Evolution #Genomics #PopGen #oyster https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13801

A haplotig in an assembly occurs when a single region is split into two places in the reference. We had them everywhere in our first eastern oyster assembly! This causes a bimodal distribution of coverage. This study shows how haplotigs affect pop gen inference and how to improve the reference by masking haplotigs.

I also made an Open Science Framework repository which contains data and an open and reproducible analyses, including the patching the figures together. The OSF site is
https://osf.io/9sm76/.
The masked assembly is @ https://doi.org/10.5281/zenodo.7799622
Please reach out with any questions!
Haplotig-masked Eastern Oyster Genome

Hosted on the Open Science Framework

OSF

We had a lot of SNPs and used strict filtering, and I suspect these effects are more pronounced with higher tolerances for missing data.

The paper itself is paywalled (but see free link above), but the nearly identical preprint is @ https://doi.org/10.1101/2022.08.29.505626

For those of you in #conservation #genomics, we show that haplotigs reduce SNP discovery and estimates of nucleotide diversity. Haplotigs also affect estimates of population structure and outlier detection, but the impacts are more nuanced.