Stephan Nothjunge

84 Followers
70 Following
22 Posts
AppSpecialist NGS automation @Hamilton
based in Basel, CH
interested in genomics, epigenetics, lab automation, cycling, mountains, nature and much more
Anyone suggestions for a digital lab journal? Tips? Approx. costs? Many thanks in advance ✌️📝🧬
When no mRNA is expressed from an enhancer in given a cell type, how to directly measure the reporter expression? Single-cell quantitative expression reporters (scQers) developed by Shendure lab can solve this problem. Key designs: 1) A dual RNA cassette decouples the detection and quantification (oBC for detection and mBC for quantification). 2) oBC are circularized Pol III transcribed barcodes which expressed >150-fold higher steady-state levels. #singlecell #devbio
https://www.biorxiv.org/content/10.1101/2022.12.10.519236v1
Noot on Twitter

“1/ UV Transilluminator below $10 V2: A #diybio update #labonthecheap”

Twitter

RT @[email protected]

Proud to see @[email protected] @[email protected] @[email protected]'s paper out @[email protected]!
We used live-cell imaging and polymer simulations to estimate chromosome looping dynamics within a TAD, and how it is controlled by cohesin and CTCF. https://rdcu.be/c02IQ 1/9

🐦🔗: https://twitter.com/LucaGiorgetti/status/1599850597900988419

Reih Dich ein in die Millionärsfreiheitsfront!

https://youtu.be/PjguNK5HiAA

CEO Reiser & Ton Scheine Erben - "Macht kaputt was euch kaputt macht / Die Millionärfreiheitsfront"

YouTube
@janboehm sehr sehr sehr feines Cover! Und ebenso gute Sendung. Keep up the good work, denn der Kampf geht weiter 😂
So, the European Union has set up its own Mastodon instance, EU Voice, as an official channel/platform for all its many institutions - what a great initiative https://social.network.europa.eu/about
#TwitterMigration #europe #Diplomacy #Transparency
EU Voice

EU Voice is the official ActivityPub platform of the EU institutions. With EU Video, it is part of an alternative social media pilot proposed and provided by the European Data Protection Supervisor.

Mastodon hosted on social.network.europa.eu

So genomic.social has been active for 20 days. How are you finding it? does anyone have any feedback?

Once we have been up for a month I'll give some more detailed cost breakdowns and detailed information about the instance. I'll share my rough modelling for the future too (as it will be a tad clearer with a month of data)

Anyway, thanks for joining and thanks to those of you who donated and the mods who help me run the place.

RT @IgorUlitsky
Are there still scientists in here? If so – a question.
For what % of the proteins in a cell line, if you tag their endogenous locus with a GFP (one allele), the GFP levels will be high enough to be seen/sorted by FACS? Top 100 most abundant? 1K? 5K? Can extrapolate from FPKM?
NEW BARTMAN LAB WEBSITE JUST DROPPED: https://bartmanlabpenn.squarespace.com/
Techs/staff scientists/postdocs/students, come work with us in 2023!
(pls retoot =)
the Bartman lab

the Bartman lab