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Here is the final version of our study dissecting in vivo, how regulation of Sox2 by its many distal enhancers interplays with CTCF loops throughout development.
Led by @[email protected]
https://www.nature.com/articles/s41588-022-01295-6
🐦🔗: https://twitter.com/Nuclear_Rocha/status/1620099669618753536
Enhancer–promoter interactions can bypass CTCF-mediated boundaries and contribute to phenotypic robustness - Nature Genetics
Genetic manipulation of the Sox2 locus in mice shows that gene activation by distal enhancers does not require CTCF-mediated loops and can occur across ectopic CTCF-mediated boundaries. The ability to bypass CTCF boundaries varies with their insulation strength and the tissue-specific enhancers responsible for activation.

