| #glycotime |
A spoonful of sugar #18 🍬
Plant receptor kinases translate and pass on chemical messages as part of many key biological processes. A new fabulous cryo-EM structure of the active complex between the kinase MIK2, the immunomodulatory peptide SCOOP and the co-receptor BAK1 shows how its binding hinges on N-glycosylation (https://doi.org/10.1038/s41477-024-01841-6)
You can rebuilt the fully glycosylated structure of the complex with https://glycoshape.org with some fancy-plant N-glycans 
A spoonful of sugar #17 🍬
Everyone needs a bit of sugar today, here is a treat
The oligosaccharyltransferase, or OST (EC 2.4.1.119), is a key enzyme that functionalizes nascent proteins with sugars called N-glycans
The 3D structure of this magnificent molecular machine has been solved by cryo-EM by Ana Ramirez et al. (PDB 8AGC) https://doi.org/10.1038/s41467-022-35067-x
Be like OST and plant one of the 195 N-glycans 🌱 online on your protein 3D structure with https://glycoshape.org
A spoonful of sugar #16 🍬
How does glycosylation affect not only structure and function of proteins, but also binding specificity?
In a new paper fresh off the #bioRxiv press https://doi.org/10.1101/2024.10.24.620132 in collaboration with Nicki Packer, Len Harrison and Ethan Goddard-Borger, we explain how glycosylation of CD52 (reconstructed with https://glycoshape.org) determines:
1) the peptide's conformation,
2) binding to the HMGB1 Box B and only then
3) to SIGLEC-10
BIG MASSIVE THANK YOU 👏 to all the beta testers around the world. Happy glycosylating! 
4/6 GlycoShape (https://glycoshape.org) is an OA database and toolbox that allows you to restore the 3D structure of glycoproteins with a fast and accurate algorithm named Re-Glyco.
The database counts > 550 glycans to date, largely from the human glycome with examples from all species. For an overview check out the figure below ⬇️
2/6 What you see through a camera lens depends on the shutter speed.
Yet we all know that hummingbirds do have wings, and that these are precisely shaped to allow the fast and complex dynamics crucial to their ability to hover and feed on nectar
Some exciting #glycotime to start the week!
GlycoShape is fresh off the press today on Nature Methods https://www.nature.com/articles/s41592-024-02464-7
Check out the short thread below 1/6
GlycoShape is an open-access web-based platform designed to supplement three-dimensional glycoprotein structures with missing structural information on glycans. To link them, the Re-Glyco algorithm evaluates the steric complementarity of glycans using their conformational ensemble with the protein surface.
The Nobel Prize in Chemistry 2024 goes to David Baker (1/2) and Demis Hassabis and John Jumper (1/2) for protein structure design and prediction from sequence with AI
https://www.nobelprize.org/prizes/chemistry/2024/summary/
Congratulations to all very worthy winners from all of us! 👏 🥳
As a reminder, just add sugars with http://glycoshape.org
