Mastodawn

Run two reactions on related substrates in parallel. Ran the column for the first on Friday, everything looked good, so set-up step 2 over the weekend.

Ran the column on the second reaction this morning. No peaks. The TLC had spots! The reactions were the same scale! tears hair out

(at least it looked like step 2 on the first reaction worked, but that wasn't really in doubt since I've done it before, step 1 was a different protocol)

#LabLife #Chemistry #ChemiVerse

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Sam Levine 18h ago

Re-ran the reaction in question and managed to do an aqueous work-up and the column for it today (did both collect all and turned up the gain 5 fold on the absorbance monitoring). Submitted an NMR sample, but instrument backlog means I won't have data for a couple of hours, so time to go home (I don't want to set-up step 2 without confirmation that this material is at least likely to be the desired product of step 1).