PuckerLabInterested in the genetic secrets of your favorite plant?
A genome sequence might reveal them. We wrote down a workflow from DNA extraction & sequencing to genome sequence assembly & annotation. It includes a hands-on guide with example commands.
DeepDNA@PuckerLab Great workflow overview! The gap between raw reads and a publishable assembly has shrunk dramatically. ONT and PacBio HiFi now give near chromosome-level assemblies with fairly straightforward pipelines. The real bottleneck has shifted from assembly to annotation and functional interpretation.