El Duvelle NeuroIf you want to study #HippocampalReplay... Use ephys, not #CalciumImaging!!
(Calcium imaging doesn't detect single spikes well, but replay mostly involves single spikes)
#Neuroscience #SpatialCognition #Hippocampus
Oh and also.. Replay happens during #SWRs or at least "large, irregular activity" state. If you cannot access the #LFP as is the case with Calcium Imaging.. then you can't know if what you're recording is replay or #ThetaSequences!
Ko-Fan Chen 陳克帆@elduvelle_neuro I was wondering last week, why those voltage sensors (like arclight sorry I am not behind with tech ) don't seem to offer the promise, or am I wrong? I do feel with #connnectome catching up we needs a way to access functional connectivity with temp resolution of ephy but better spatially? #neuroscience #LFP
@kofanchen I also don't know enough about the imaging techniques or advancements in those to say. But there are quite good techniques that combine optogenetic tracing and ephys, for example like in this study: Selective information routing by ventral hippocampal CA1 projection neurons - very small yield though, but I'm sure if you combined this with neuropixels recordings you could get the best of both worlds?
@elduvelle_neuro neuropixel still way too big for #drosophila brain? But guess good enough for rodents?
@kofanchen Yes definitely fine for rodents! I'm not sure what is the smallest organism they've been used with.. I was thinking maybe they used NP in this butterfly study (Neural representation of goal direction in the monarch butterfly brain but no it's just tetrodes..
Neural representation of goal direction in the monarch butterfly brain - Nature Communications
Neural coding of goal direction remains unclear in insects. Here, the authors describe goal-direction neurons in the monarch butterfly brain that specifically encode the insect’s desired flight direction during spatial orientation.