Toxoplasma gondii rhoptry discharge factor 3 is essential for invasion and microtubule-associated vesicle biogenesis

Regulated secretion of rhoptry factors is essential for host cell invasion by Apicomplexan parasites. This study identifies the protein rhoptry discharge factor 3 (RDF3) of Toxoplasma gondii as crucial for invasion, by priming microtubule-associated vesicles for rhoptry discharge.