Sebastian WendelNov 29, 2023
@relet what a great idea to display #microscopy images with #GIS tooling https://setzkasten.relet.net/ . An awesome #OSS project https://github.com/relet/setzkasten Away from the software, what hardware do you use to create such high resolution images?
Setzkasten

Show threadThomas Hirsch [moved]

@swendel Hey, thanks a lot.

The hardware is pretty basic, if you are patient. I started with an old Samsung S9, a basic #microscope with movable platform (Bresser Erudit DLX) and a phone holder for the eyepiece.

Stacking happens in #enfuse using
https://github.com/relet/rolling-focus (but after gaining confidence I dropped the live preview in exchange for speed)

Stitching can be done with #hugin, though #PTGui is nicer and I like to support the project.

1/3

GitHub - relet/rolling-focus: live preview focus stacking for microscopy and android

live preview focus stacking for microscopy and android - GitHub - relet/rolling-focus: live preview focus stacking for microscopy and android

GitHub
Nov 29, 2023 at 3:52pmTusky
Show threadThomas Hirsch [moved]Nov 29, 2023

@swendel

I built some more #automation around this basic setup, which is an #Arduino that could in theory control the XYZ axes of the microscope.

Unfortunately I found that my object table moves the XY controls along with the table, so I'm currently stuck with automating Z.

Still dreaming of a proper table with stationary control. Or maybe an actual controller...

2/3

Show threadThomas Hirsch [moved]Nov 29, 2023

@swendel

This is the current state of things. #macgyver'ed everything. Of course, if it worked the way I had intended, I would consider 3d printing the fixtures. 3/3