aryunni Aug 5, 2023
I’m starting #scRNAseq and IMC #experiments soon and I’m distraught by the fact that I have zero dry-lab experience 😫😩😭
Show threadEli Roberson (he/him)Aug 5, 2023

@aryunni Yes... come to the data dark side *cackles in dimensionality reduction*

It's survivable. The question will be if you *like* doing it. If you end up hating it you probably shouldn't keep doing it.

Show threadaryunni Aug 5, 2023
@thatdnaguy I’ve been doing pie charts to visualize BCR-CDR3 sequencing using Excel !!! It’s doable but it’s not practical (and also silly *bang head on table*) So I think I will not only *like* but would LOVE to come to the „dark side“ 🙃
Show threadEli Roberson (he/him)Aug 5, 2023

@aryunni yeah I've been bitten by doing some things "manually" in Excel and the like before. It isn't that coding is error proof. Far from it. But you *should* have a written record of the steps. Need to repeat it two years later? You can. Think something is amiss? Someone else can run it.

Pie charts are pretty common for those things. But after seeing enough about how people can't judge proportions in pie charts I just can't bring myself to use them. 🤓

Show threadaryunni Aug 5, 2023
@thatdnaguy these are useful tips and advice for newbie like moi🤓 thank you 🙏 p/s: I will rethink the pie charts and just might report back😉
aryunni Aug 6, 2023
Show threadEli Roberson (he/him)
@aryunni do what works until you find a better alternative. What I always end up telling people is that often there isn't a right way to do it. But sometimes there are some very wrong ways. We're all just doing the best we can.
Aug 5, 2023 at 12:59pmWeb