Dagmar Iber

As theoretician, I am excited to share the 1st experimental protocols paper from my lab
"Time-lapse and cleared imaging of mouse embryonic lung explants to study 3D cell morphology and topology dynamics"

https://star-protocols.cell.com/protocols/2552

Thx @starprotocols for inviting this protocol!!

Cell Press: STAR Protocols

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Mar 24, 2023 at 8:08amWeb
Show threadDagmar IberMar 24, 2023

The protocol provides the step-by-step experimental methods for our @eLife paper "3D cell neighbour dynamics in growing pseudostratified epithelia" https://elifesciences.org/articles/68135.

See also our Tweetorial:
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RT @DagmarIber
How many neighbours does an epithelial cell have? And how stable are these interactions over time? #epithelialcell #topology #LSFM #SPIM

Check out our latest pre-print: https://www.biorxiv.org/content/10.…
https://twitter.com/DagmarIber/status/1368806675495452672

3D cell neighbour dynamics in growing pseudostratified epithelia

The 3D shape and dynamic organization of epithelial cells are far more complex than previously believed, but can be explained with simple physical principles based on lateral surface energy minimisation.

eLife
Show threadDagmar IberMar 24, 2023
Kudos to @HaroldGomezETH who developed the protocol. Harold, a trained computer scientist, quickly realised in his PhD that #image #segmentation would become much easier if the data quality improved - and went to the lab himself to optimise the experimental technique.