The Level of Ubiquitin Conjugates in Murine Blood Serum: Relevance to Radioprotection and Age-Related Variability - Cytology and Genetics

Abstract Human exposure to radiation, such as cosmic and earth background radiation, industrial background radiation, as well as radiation cause by various human activities is know to trigger various intra and extra-cellular responses. It is also known that the radiation response and sensitivity is age-dependent. Ubiquitin conjugates in murine blood serum were analized under normal and pathological conditions caused by irradiation across different age groups. Nonlinear white mice Mus Musculus were used for tests. The source of radiation was 137Cs with dose rate 1Gy/min, due exposure 5 min (LD50 5Gy) executed using the “GUPOS-3M” gamma facility. Using immunoblotting for measurement data was statistically analyzed to unveil changes in ubiquitin conjugate levels following ionizing radiation and intraperitoneal ubiquitin administration. Age-related variability in ubiquitin conjugates levels is identified, shedding light on ubiquitin injection role in modulating radiation responses across age groups. These findings hold implications for cellular biology and clinical radiation therapy, offering hope for future therapeutic interventions and enhanced radiation therapy outcomes.