Happy to share updated Nano3P-seq #protocol that can sequence any #RNA molecule from its 3’ end, without the need for PCR amplification or RNA adapter ligation. New version supports #nanopore R10 chemistry and replaces deprecated #TGIRT with #Induro.
We released new tool, PolyTailor, accurately estimating transcript abundances, #tail #lengths and content information, while capturing both coding and non-coding RNA biotypes.

https://www.biorxiv.org/content/10.1101/2024.11.20.624491v1
https://github.com/novoalab/polyTailor