Direct Salmonella injection into enteroid cells allows the study of host–pathogen interactions in the cytosol with high spatiotemporal resolution

A previous study in PLOS Biology developed a FluidFM-based approach to inject organelles into living cells. Using this technology, this study shows that establishment of a Salmonella Typhimurium replicative niche in the cytosol of enteroid cells, when uncoupled from invasion, does not require flagellin or type III secretion system effectors.