Royer Lab at CZ Biohub

@royerlab
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How do organisms develop from a single cell?

The Royer Lab is a pluridisciplinary team of computer scientists, optical engineers, and biologists. Our goal is to build a time-resolved, multidimensional atlas of vertebrate development using zebrafish as a model organism. To attain this goal, we design, build, and implement novel state-of-the-art light-sheet microscopes, and employ and also design deep learning–based image processing and analysis algorithms.

Show threadRoyer Lab at CZ BiohubMar 8, 2023
Many thanks to the @slschmid_CZB, Joe DeRisi, and @StephenQuake for mentorship in the past 5 years! and to the donors of the @czbiohub for their support.
Show threadRoyer Lab at CZ BiohubMar 8, 2023
Plus to our wonderful collaborators, Olivier Pourquie, @Mattia__Serra, @SreejithS_, @haesleinhuepf, @AlexandreDizeux 24/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
All the @czbiohub contributors @mikeborjatweets, Sheryl Paul, Honey Mekonen, Angela Detweiler, @liilii_tweet, Erin McGeever, @Bin_YANG_Optics, @hoover_zhao, @yang_gp, @kyleawayan Samuel D’Souza, @Adrian_Jacobo, @keirballa, Rafael Gómez-Sjöberg, Greg Huber, Norma Neff, @drAOPisco
Show threadRoyer Lab at CZ BiohubMar 8, 2023
This work would not have been possible without the amazing #team at @czbiohub sf, in particular, @Merlin_Lange who led this work, and a special shoutout to @ale_agranados, @Shruthi94Vijay, @jobragantini, and @Sarah_E_Ancheta for there wonderful contribution. 22/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
Thanks to last-minute help from @stardazed0 and the admirably twitter-less Jeremy Maitin-Shepard we managed to integrate github.com/google/neuroglancer in zebrahub.org/imaging Check it out! It's amazingly fast! 21/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
We are still trying to understand all this, and have some ideas in the preprint. We expect and hope for a robust and enthusiastic discussion with the community and welcome feedback, ideas, and suggestions! Reach out at [email protected] and [email protected] 20/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
To understand the relevant tissue kinematics, we applied the recently developed dynamic morphoskeletons framework from @Mattia__Serra et al. and found that pluripotent NMPs coincide with a strong repeller structure. For more details on the theory check the preprint 😉 19/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
Using the in-vivo experiment we can reconstruct the track of a single pluripotent presumptive NMP! 18/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
'in silico' fate mapping is great, but there is nothing better than a real experiment. Next, we performed similar in vivo photo-manipulation experiments directly in our multi-view light-sheet microscope. Again we find the same fate restriction. 17/n
Show threadRoyer Lab at CZ BiohubMar 8, 2023
Using the high-resolution image-based cell tracking together with our @napari_imaging plugin for in silico fate mapping we confirmed the NMP state transition in pluripotency. 16/n