I want to give a shout out to Biomart, which was incredibly fast in helping convert a Kalisto from raw reads to TPM
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Joel Rosenbaum
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I should mention that it's an orthologous CDS (using a query from a related species, the map is on the ortholog genome).
Question for the #genomics community: When you perform a web tblast against a (eukaryotic) genome assembly, NCBI will produce output that joins together your query to give an approximate map of the CDS, with intron/exon boundaries.
But you can't download this output, which is quite aggravating. Is there a way to get the same thing out of BLAST+ (or at least something that can be easily strung together with Biopython)
Question for the folks here; does anyone remember Trevigel? I'm assuming that this product has been discontinued for some time, but if it's still out there, I'd love to know:
Wild geraniums, on the back side of their bloom period but still stunning this year:
Tagging @SRDas here.
Why is the BCA-Cu(I) complex so stable? It is essentially irreversible even in aqueous solution, even in the presence of oxygen.
Question for you guys: why don't we euthanize mice (or other lab animals) with N2?
Is there a way to get tabulated data for the following paper? I simply want a positional substitution matrix and it doesn't look like it's provided or linked anywhere with this article:
Local fitness landscape of the green fluorescent protein - Nature
Comprehensive genotype–phenotype mapping of the green fluorescent protein shows that the local fitness peak is narrow, shaped by a high prevalence of epistatic interactions, providing for the loss of fluorescence when the joint effect of mutations exceeds a threshold.
It only took me a week to figure out some pretty basic calculations involving α and its effect on an association constant
please clap